Portable, Quantitative Detection of Bacillus Bacterial Spores Using Surface-Enhanced Raman Scattering
Portable, Quantitative Detection of Bacillus Bacterial Spores Using Surface-Enhanced Raman Scattering
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DOI：
10.1021/ac303657k
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发表时间：
2013-03-19
影响因子：
7.4
通讯作者：
Goodacre, Royston
中科院分区：
文献类型：
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作者：
Cowcher, David P.;Xu, Yun;Goodacre, Royston
便携式快速检测病原菌如芽孢杆菌是非常需要的食品生产中的安全和在目前的生物恐怖主义的风险增加。表面增强拉曼散射（Sers）技术由于其分析速度快、灵敏度高而成为细菌检测的首选分析技术。然而，在寻求提供最低检测限的方法时，目前的研究倾向于高度共焦的、基于显微镜的分析，这需要有点笨重的仪器和精确合成Sers基底。相比之下，在这项研究中，我们已经改进了Sers细菌分析使用银胶体基板，这是很容易和廉价的批量合成，我们将证明允许使用便携式仪器分析。所有分析均重复进行三次，以评估该方法的重现性，该方法非常出色。我们证明，Sers是能够检测和快速定量的吡啶二羧酸（DPA）的芽孢杆菌孢子的生物标志物在5 ppB（29.9 nM）的水平，这是显着低于那些先前报道的Sers和远低于104 B的感染剂量。炭疽细胞用于吸入性炭疽。最后，我们展示了多变量数据分析的潜力，以提高检测水平，在复杂的DPA提取物从活孢子。
Portable rapid detection of pathogenic bacteria such as Bacillus is highly desirable for safety in food manufacture and under the current heightened risk of biological terrorism. Surface-enhanced Raman scattering (SERS) is becoming the preferred analytical technique for bacterial detection, due to its speed of analysis and high sensitivity. However in seeking methods offering the lowest limits of detection, the current research has tended toward highly confocal, microscopy-based analysis, which requires somewhat bulky instrumentation and precisely synthesized SERS substrates. By contrast, in this study we have improved SERS for bacterial analyses using silver colloidal substrates, which are easily and cheaply synthesized in bulk, and which we shall demonstrate permit analysis using portable instrumentation. All analyses were conducted in triplicate to assess the reproducibility of this approach, which was excellent. We demonstrate that SERS is able to detect and quantify rapidly the dipicolinate (DPA) biomarker for Bacillus spores at 5 ppb (29.9 nM) levels which are significantly lower than those previously reported for SERS and well below the infective dose of 104 B. anthracis cells for inhalation anthrax. Finally we show the potential of multivariate data analysis to improve detection levels in complex DPA extracts from viable spores.