Regulation of renal epithelial cell endocytosis of calcium oxalate monohydrate crystals.
Regulation of renal epithelial cell endocytosis of calcium oxalate monohydrate crystals.
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DOI：
10.1152/ajprenal.1993.264.5.f800
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发表时间：
1993-05
期刊：
影响因子：
--
通讯作者：
J. Lieske;F. Toback
中科院分区：
文献类型：
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作者：
J. Lieske;F. Toback
肾结石的早期症状是什么？最常见的晶体在肾结石，草酸钙一水合物（COM），当添加到培养的猴肾上皮细胞（BSC-1线），被内化的19%的细胞30分钟后。COM晶体内吞增强血清，ADP，和表皮生长因子，这是有效的有丝分裂原，这些细胞。COM晶体的内吞作用受到多种分子的抑制，包括Tamm-Horsfall糖蛋白（THP），四肽甘氨酸-甘氨酸-天冬氨酸-丝氨酸，纤连蛋白，转化生长因子-β 2和肝素。THP、纤连蛋白或肝素抑制内吞作用的能力是通过这些分子与细胞的相互作用介导的，而不是通过包覆晶体。因此，COM晶体的肾上皮细胞内吞作用可以由不同的分子调节，包括THP，在人尿中发现的最常见的蛋白质。晶体内吞和随后的细胞反应可能是肾结石的重要致病步骤。
The earliest events in the formation of kidney stones are unknown. The most common crystal in kidney stones, calcium oxalate monohydrate (COM), when added to cultures of monkey kidney epithelial cells (BSC-1 line), was internalized by 19% of the cells after 30 min. COM crystal endocytosis was enhanced by serum, ADP, and epidermal growth factor, which are potent mitogens for these cells. Endocytosis of COM crystals was inhibited by diverse molecules including Tamm-Horsfall glycoprotein (THP), the tetrapeptide arginine-glycine-aspartic acid-serine, fibronectin, transforming growth factor-beta 2, and heparin. The capacity of THP, fibronectin, or heparin to inhibit endocytosis was mediated by an interaction of these molecules with cells, not by coating the crystals. Thus renal epithelial cell endocytosis of COM crystals can be regulated by diverse molecules including THP, the most common protein found in human urine. Crystal endocytosis and subsequent cellular responses could be important pathogenic steps in nephrolithiasis.