Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls
Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls
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DOI：
10.1111/j.1462-2920.2004.00696.x
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发表时间：
2005-03-01
影响因子：
5.1
通讯作者：
Verstraete, W
中科院分区：
文献类型：
--
作者：
De Windt, W;Aelterman, P;Verstraete, W
通过希瓦氏菌（Shewanella oneidensis）MR-1和自聚集突变体（COAG）的细胞对可溶性Pd（II）的微生物还原导致钯Pd（0）纳米颗粒沉淀在细胞壁上和周质空间内（bioPd）。由于生物吸附和随后的生物还原的Pd（II）与H-2，甲酸，乳酸，丙酮酸或乙醇作为电子供体，回收率高于90%的Pd与生物质可以得到。由此获得的bioPd（0）纳米颗粒具有还原性脱卤的能力，多氯联苯（PCB）同系物在水和沉积物基质。在浓度高达1000 mg Pd（II）I（-1）的测定中观察到生物还原，可溶性Pd（II）的消耗为77.4%或更高。在28 ℃下，浓度为1 mg l（-1）的PCB 21（2，3，4-氯联苯）在5小时内减少了90%以上，同时还生成了脱氯产物PCB 5（2，3-氯联苯）和PCB 1（2-氯联苯）。S. oneidensis细胞与受多氯联苯同系物混合物污染的沉积物样品混合，导致在28 ℃下48小时内吸附到受污染沉积物基质的高氯化和低氯化多氯联苯同系物的脱氯。每升50毫克的bioPd产生的催化活性与500毫克l（-1）商业Pd（0）粉末相当。高反应性的50毫克升（-1）bioPd在土壤悬浮液中反映在7个最有毒的多氯联苯的总和减少到其初始浓度的27%。
Microbial reduction of soluble Pd(II) by cells of Shewanella oneidensis MR-1 and of an autoaggregating mutant (COAG) resulted in precipitation of palladium Pd(0) nanoparticles on the cell wall and inside the periplasmic space (bioPd). As a result of biosorption and subsequent bioreduction of Pd(II) with H-2, formate, lactate, pyruvate or ethanol as electron donors, recoveries higher than 90% of Pd associated with biomass could be obtained. The bioPd(0) nanoparticles thus obtained had the ability to reductively dehalogenate polychlorinated biphenyl (PCB) congeners in aqueous and sediment matrices. Bioreduction was observed in assays with concentrations up to 1000 mg Pd(II) l(-1) with depletion of soluble Pd(II) of 77.4% and higher. More than 90% decrease of PCB 21 (2,3,4-chloro biphenyl) coupled to formation of its dechlorination products PCB 5 (2,3-chloro biphenyl) and PCB 1 (2-chloro biphenyl) was obtained at a concentration of 1 mg l(-1) within 5 h at 28degreesC. Bioreductive precipitation of bioPd by S. oneidensis cells mixed with sediment samples contaminated with a mixture of PCB congeners, resulted in dechlorination of both highly and lightly chlorinated PCB congeners adsorbed to the contaminated sediment matrix within 48 h at 28degreesC. Fifty milligrams per litre of bioPd resulted in a catalytic activity that was comparable to 500 mg l(-1) commercial Pd(0) powder. The high reactivity of 50 mg l(-1) bioPd in the soil suspension was reflected in the reduction of the sum of seven most toxic PCBs to 27% of their initial concentration.