Survey on the PABC recognition motif PAM2
Survey on the PABC recognition motif PAM2
复制标题
DOI：
10.1016/j.bbrc.2004.02.024
复制
发表时间：
2004-03-26
影响因子：
3.1
通讯作者：
Lengauer, T
中科院分区：
文献类型：
--
作者：
Albrecht, M;Lengauer, T
PABP 相互作用基序 PAM2 已在各种真核蛋白中被鉴定为 PABC 结构域的重要结合位点。该结构域包含在聚腺苷酸结合蛋白 PABP 和泛素蛋白连接酶 HYD 的同源物中。尽管 PAM2 基序很重要，但对其在不同蛋白质中发生情况的全面分析却一直缺失。通过迭代序列图谱搜索，我们获得了携带 PAM2 基序的大量蛋白质。我们讨论它们的功能背景和结构域结构，通常由 RNA 结合结构域组成。我们的 PAM2 基序蛋白列表包括 eRF3/GSPT1/2、PAIP1/2、Tob1/2、Ataxin-2、RBP37、RBP1、Blackjack、HELZ、TPRD、USP10、ERD15、C1D4.14 和病毒蛋白酶 P29 的真核同源物。在尚未表征的蛋白质中鉴定 PAM2 基序可以为其细胞功能和潜在的相互作用伙伴提供有价值的提示，并建议进一步的实验。同样引人注目的是，PAM2 基序似乎仅出现在球状蛋白结构域之外。 (C) 2004 Elsevier Inc. 保留所有权利。
The PABP-interacting motif PAM2 has been identified in various eukaryotic proteins as an important binding site for the PABC domain. This domain is contained in homologs of the poly(A)-binding protein PABP and the ubiquitin-protein ligase HYD. Despite the importance of the PAM2 motif, a comprehensive analysis of its occurrence in different proteins has been missing. Using iterated sequence profile searches, we obtained an extensive list of proteins carrying the PAM2 motif. We discuss their functional context and domain architecture, which often consists of RNA-binding domains. Our list of PAM2 motif proteins includes eukaryotic homologs of eRF3/GSPT1/2, PAIP1/2, Tob1/2, Ataxin-2, RBP37, RBP1, Blackjack, HELZ, TPRD, USP10, ERD15, C1D4.14, and the viral protease P29. The identification of the PAM2 motif in as yet uncharacterized proteins can give valuable hints with respect to their cellular function and potential interaction partners and suggests further experimentation. It is also striking that the PAM2 motif appears to occur solely outside globular protein domains. (C) 2004 Elsevier Inc. All rights reserved.