In vitro simulation of the effect of peritoneal dialysis solution on mesothelial cells.
In vitro simulation of the effect of peritoneal dialysis solution on mesothelial cells.
复制标题
腹膜透析液对间皮细胞影响的体外模拟。
DOI：
10.1016/s0272-6386(97)90202-x
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发表时间：
1997
影响因子：
13.2
通讯作者：
D. Oreopoulos
中科院分区：
文献类型：
--
作者：
A. Bręborowicz;H. Rodela;J. Karoń;Leo Martis;D. Oreopoulos
所有先前的腹膜透析液体外生物相容性试验均表明，这些透析液对腹膜间皮功能具有抑制作用。本报告提供了体外实验的结果，该实验旨在研究透析液（Dianeal 1.36和Dianeal 3.86;巴克斯特，圆湖，IL）在模拟这些溶液在腹膜腔中的体内状态的条件下对间皮细胞功能的影响。因此，细胞最初仅暴露于未使用的液体，随后用来自持续非卧床腹膜透析患者的合并流出透析液逐渐稀释（超过4小时）。在接下来的20小时内，将细胞在未使用的液体（10%vol/vol）和透析液流出物（90%vol/vol）的混合物中孵育。与单独暴露于透析液流出物（对照）相比，在这种条件下暴露于透析液的间皮细胞变为活化细胞。因此，在间皮细胞暴露于透析液期间，间皮细胞对所有供试物质的合成均得到增强：白细胞介素-6：Dianeal 1.36，+257%; Dianeal 3.86，+181%（均P < 0.05）;透明质酸：Dianeal 1.36，+72%; Dianeal 3.86，+63%组织型纤溶酶原激活剂：Dianeal 3.86，+33%（P < 0.05）;纤溶酶原激活剂/抑制剂-1：Dianeal 1.36，+28%; Dianeal 3.86，+38%（P < 0.05）。我们的研究结果表明，腹膜间皮细胞被激活时，它是暴露于酸性，高渗透析液稀释的透析液流出物，在某种程度上，模仿在体内的变化，这些解决方案在其腹膜内停留。
All previous in vitro biocompatibility tests of peritoneal dialysis fluids have shown that these have inhibitory effects on the function of peritoneal mesothelium. This report presents results from in vitro experiments performed to study the effect of dialysis fluids (Dianeal 1.36 and Dianeal 3.86; Baxter, Round Lake, IL) on the function of mesothelial cells under conditions that simulate the in vivo state of these solutions in the peritoneal cavity. Thus, cells were initially exposed only to the unused fluids that were thereafter gradually diluted (over 4 hours) with pooled effluent dialysate from continuous ambulatory peritoneal dialysis patients. During the following 20 hours, cells were incubated in a mixture of unused fluid (10% vol/vol) and dialysate effluent (90% vol/vol). The mesothelial cells exposed to dialysis fluids under such conditions became activated cells compared with exposed to dialysate effluent (control) alone. Thus, synthesis by mesothelial cells of all tested substances was enhanced during exposure of the mesothelium to the dialysis fluids: interleukin-6: Dianeal 1.36, +257%; Dianeal 3.86, +181% (both P < 0.05); hyaluronic acid: Dianeal 1.36, +72%; Dianeal 3.86, +63% (both P < 0.05); tissue plasminogen activator: Dianeal 3.86, +33% (P < 0.05); and plasminogen activator/inhibitor-1: Dianeal 1.36, +28%; Dianeal 3.86, +38% (both P < 0.05). Our results show that the peritoneal mesothelium becomes activated when it is exposed to acidic, hyperosmotic dialysis fluids diluted with the dialysate effluent, in a manner that imitates the in vivo changes in these solutions during their intraperitoneal dwell.