Rapid simultaneous determination of ephedrines, amphetamines, cocaine, cocaine metabolites, and opiates in human urine by GC-MS.
Rapid simultaneous determination of ephedrines, amphetamines, cocaine, cocaine metabolites, and opiates in human urine by GC-MS.
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DOI：
10.1016/j.jpba.2006.06.031
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发表时间：
2007-01
影响因子：
3.4
通讯作者：
Takeshi Saito;Hiroyasu Mase;S. Takeichi;S. Inokuchi
中科院分区：
文献类型：
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作者：
Takeshi Saito;Hiroyasu Mase;S. Takeichi;S. Inokuchi
本文提出了一种简单，灵敏的气相色谱-质谱法同时测定和定量的人尿中的可卡因，安非他明，可卡因，可卡因代谢物，阿片类药物的色谱程序。该方法包括在氘代内标物存在下的酶水解、液-液萃取以及用五氟丙酸酐和五氟丙醇衍生化。各化合物的平均回收率为65.8%或更高。通过使用2 mL样品体积测定的每种化合物的检测限范围为5至50 ng/mL。以甲基苯丙胺-d4和MDMA-d5为内标物，所有化合物的线性范围均为1000 ng/mL。该方法已成功应用于甲基苯丙胺、海洛因等毒品的尿样分析。
This paper presents a simple and sensitive chromatographic procedure for the simultaneous determination and quantification of ephedrines, amphetamines, cocaine, cocaine metabolites, and opiates in human urine by gas chromatography–mass spectrometry. This method involves enzyme hydrolysis in the presence of a deuterated internal standard, liquid–liquid extraction, and derivatization with pentafluoropropionic anhydride and pentafluoropropanol. The recovery of each compound averaged at 65.8% or more. The limits of detection determined for each compound by using a 2-mL sample volume ranged from 5 to 50ng/mL. The calibration curves were linear to 1000ng/mL for all compounds when determined using methamphetamine-d4and MDMA-d5as internal standards. This method was successfully applied for the analysis of urine samples suspected to contain intoxicants such as methamphetamine and heroin.