Effectiveness of egg immersion in aqueous solutions of thiamine and thiamine analogs for reducing early mortality syndrome
Effectiveness of egg immersion in aqueous solutions of thiamine and thiamine analogs for reducing early mortality syndrome
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DOI：
10.1577/h03-075.1
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发表时间：
2005-03-01
影响因子：
1.2
通讯作者：
Wolgamood, M
中科院分区：
文献类型：
--
作者：
Brown, SB;Brown, LR;Wolgamood, M
在湖鳟 Salvelinus namaycush 和银鲑 Oncorhynchus kisutch 中研究了用于治疗鲑鱼早期死亡综合征 (EMS) 的硫胺素治疗方案，以评估其疗效。需要在蛋浴中添加至少 500 mg/L 的盐酸硫胺素，才能持续提高湖鳟鱼卵中的硫胺素含量。硫胺素的吸收例如。浴液不受 5.5 至 7.5 范围内的 pH 值或 2 至 200 mg CaCO3/L 之间的水硬度的影响。当最初的硫胺素水平较低时，硫胺素的吸收较差，这表明当硫胺素水平严重降低时，当前的治疗方案可能不那么有效，并且需要更高的治疗剂量。在水硬化过程中，将鸡蛋暴露于脂溶性更强的硫胺素类似物蒜硫胺 (1,000 mg/L) 中，可使鸡蛋硫胺素水平增加 1.5-2.5 nmol/g，并且对逆转 EMS 完全有效。另一种脂溶性更高的硫胺素类似物苯磷硫胺 (100 mg/L) 可降低 EMS，但不会导致鸡蛋硫胺素含量明显增加。尽管苯磷硫胺在缓解 EMS 方面可能比硫胺素更有效，但它比盐酸硫胺素或蒜硫胺更昂贵。此外，还需要对剂量反应关系进行更彻底的检查。我们得出的结论是，蒜硫胺可以替代在蛋浴中使用硫胺素来治疗鲑鱼 EMS。
Protocols used for therapeutic thiamine treatments in salmonine early mortality syndrome (EMS) were investigated in lake trout Salvelinus namaycush and coho salmon Oncorhynchus kisutch to assess their efficacy. At least 500 mg of thiamine HCl/L added to egg baths was required to produce a sustained elevation of thiamine content in lake trout eggs. Thiamine uptake from eg. baths was not influenced by a pH ranging from 5.5 to 7.5 or by a water hardness between 2 and 200 mg CaCO3/L. There was poorer thiamine uptake when initial thiamine levels were low, suggesting that current treatment regimes may not be as effective when thiamine levels are severely depressed and that higher treatment doses are necessary. Exposure of eggs to the more lipid-soluble thiamine analog allithiamine (1,000 mg/L) during water hardening increased egg thiamine levels by 1.5-2.5 nmol/g and was completely effective at reversing EMS. Another more lipid-soluble thiamine analog, benfotiamine (100 mg/L), reduced EMS but did not produce detectable increases in egg thiamine content. Although benfotiamine may be more effective than thiamine at mitigating EMS, it is more expensive than thiamine HCl or allithiamine. In addition, there still needs to be a more thorough examination of dose-response relationships. We conclude that allithiamine is an alternative to the use of thiamine in egg baths as a therapeutic treatment for salmonid EMS.