Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus
Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus
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DOI：
10.1016/s0167-4781(98)00006-2
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发表时间：
1998-04-01
期刊：
影响因子：
--
通讯作者：
Palacios, R
中科院分区：
文献类型：
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作者：
Asturias, JA;Arilla, MC;Palacios, R
从尘螨中克隆了一种变应原基因，并进行了序列测定。变应原(Der P 10)是一种原肌球蛋白，与其他无脊椎动物原肌球蛋白有超过65%的相同残基。经一步纯化后，重组变应原的回收率为26 mg/L。重组变应原与对虾抗原肌球蛋白抗体具有较高的反应性，与尘螨过敏患者血清中的IgE反应率为5.6%。(C)1998年爱思唯尔科学公司。
The cDNA encoding an allergen from the dust mite Dermatophagoides pteronyssinus has been cloned and sequenced. The allergen (Der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. The final recovery of recombinant Der p 10 from the culture media after a single purification step was as much as 26 mg/l. The recombinant allergen is reactive to shrimp antitropomyosin IgG antibodies and has a 5.6% frequency of IgE reactivity in sera from mite-allergic patients. (C) 1998 Elsevier Science B.V.