CC Hydrolases for Biocatalysis
CC Hydrolases for Biocatalysis
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DOI：
10.1002/adsc.201300232
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发表时间：
2013-06
影响因子：
5.4
通讯作者：
Elina Siirola;A. Frank;G. Grogan;W. Kroutil
中科院分区：
文献类型：
--
作者：
Elina Siirola;A. Frank;G. Grogan;W. Kroutil
尽管CC键水解酶在自然界中分布广泛，但与c -杂原子水解酶（如脂肪酶和蛋白酶）相比，它们在生物催化领域的关注有限。然而，CC水解酶的底物范围及其对辅因子的不依赖性表明，这些酶在合成中可能具有相当大的应用潜力。此外，已知的水解酶，如红球菌（OCH）的β-二酮水解酶，可催化形成有趣的手性中间体。进一步的酶，如kynureninase和meta-cleavage product hydrolase (MhpC)，能够催化碳-碳键的形成，这表明在生物催化方面的应用比以前设想的更广泛。本文综述了CC水解酶的分布、催化特性和应用，以期对其在生物催化过程中的应用潜力进行评价。
Although CC bond hydrolases are distributed widely in Nature, they has as yet have received only limited attention in the area of biocatalysis compared to their counterpart the C-heteroatom hydrolases, such as lipases and proteases. However, the substrate range of CC hydrolases, and their non-dependence on cofactors, suggest that these enzymes may have considerable potential for applications in synthesis. In addition, hydrolases such as the β-diketone hydrolase from Rhodococcus (OCH) are known, that catalyse the formation of interesting chiral intermediates. Further enzymes, such as kynureninase and a meta-cleavage product hydrolase (MhpC), are able to catalyse carbon-carbon bond formation, suggesting wider applications in biocatalysis than previously envisaged. In this review, the distribution, catalytic characteristics and applications of CC hydrolases are described, with a view to assessing their potentialfor use in biocatalytic processes in the future.