OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity
OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity
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OsCERK1 和 OsRLCK176 在水稻先天免疫中的肽聚糖和几丁质信号传导中发挥重要作用
DOI：
10.1111/tpj.12710
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发表时间：
2014-12-01
期刊：
影响因子：
7.2
通讯作者：
Wang, Hong-Bin
中科院分区：
文献类型：
--
作者：
Ao, Ying;Li, Zhangqun;Wang, Hong-Bin
微生物相关分子模式（MAMP）触发的免疫在植物的基础抗性防御反应中起着关键作用。几丁质和肽聚糖（PGN）分别是真菌和细菌的主要分子模式。OsLYP4和OsLYP6是两种水稻溶素基序蛋白，它们作为受体感受细菌PGN和真菌几丁质。这些缺乏胞内激酶结构域的膜受体可能含有跨膜免疫信号转导的另一种组分。在这里，我们证明了水稻LysM受体样激酶OsCERK1，几丁质激发子信号通路的关键组成部分，也发挥了重要的作用，在水稻的PGN触发的免疫。OsCERK1的沉默抑制PGN诱导的（和几丁质诱导的）免疫反应，包括活性氧的产生，防御基因的表达，和胼胝质沉积，表明OsCERK1是必不可少的PGN和几丁质信号启动OsLYP4和OsLYP6。在没有PGN或几丁质的情况下，OsLYP4与OsLYP6和水稻几丁质受体几丁质寡糖激发子结合蛋白（CEBiP）相关联，并且用PGN或几丁质处理导致它们在体内解离。OsCERK1在PGN诱导下与OsLYP4或OsLYP6结合，但在几丁质处理下与OsLYP4、OsLYP6或CEBiP结合，表明存在不同模式的配体诱导的异源寡聚受体复合物。此外，受体样胞质激酶OsRLCK176在PGN和几丁质信号通路中的OsCERK1下游起作用，表明这些MAMPs共享重叠的胞内信号组分。因此，OsCERK1在水稻天然免疫中的PGN和几丁质信号传导中起双重作用，并作为衔接子与OsLYP4和OsLYP6一起参与质膜上的信号转导。
Microbe-associated molecular pattern (MAMP)-triggered immunity plays critical roles in the basal resistance defense response in plants. Chitin and peptidoglycan (PGN) are major molecular patterns for fungi and bacteria, respectively. Two rice (Oryza sativa) lysin motif-containing proteins, OsLYP4 and OsLYP6, function as receptors that sense bacterial PGN and fungal chitin. These membrane receptors, which lack intracellular kinase domains, likely contain another component for transmembrane immune signal transduction. Here, we demonstrate that the rice LysM receptor-like kinase OsCERK1, a key component of the chitin elicitor signaling pathway, also plays an important role in PGN-triggered immunity in rice. Silencing of OsCERK1 suppressed PGN-induced (and chitin-induced) immunity responses, including reactive oxygen species generation, defense gene expression, and callose deposition, indicating that OsCERK1 is essential for both PGN and chitin signaling initiated by OsLYP4 and OsLYP6. OsLYP4 associated with OsLYP6 and the rice chitin receptor chitin oligosaccharide elicitor-binding protein (CEBiP) in the absence of PGN or chitin, and treatment with PGN or chitin led to their disassociation in vivo. OsCERK1 associated with OsLYP4 or OsLYP6 when induced by PGN but it associated with OsLYP4, OsLYP6, or CEBiP under chitin treatment, suggesting the presence of different patterns of ligand-induced heterooligomeric receptor complexes. Furthermore, the receptor-like cytoplasmic kinase OsRLCK176 functions downstream of OsCERK1 in the PGN and chitin signaling pathways, suggesting that these MAMPs share overlapping intracellular signaling components. Therefore, OsCERK1 plays dual roles in PGN and chitin signaling in rice innate immunity and as an adaptor involved in signal transduction at the plasma membrane in conjunction with OsLYP4 and OsLYP6.