Impression cytology of the conjunctival epithelium in patients with vernal conjunctivitis
Impression cytology of the conjunctival epithelium in patients with vernal conjunctivitis
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DOI：
10.1038/eye.1996.12
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发表时间：
1996-01-01
期刊：
影响因子：
3.9
通讯作者：
Ferreri, G
中科院分区：
文献类型：
--
作者：
Aragona, P;Romeo, GF;Ferreri, G
用结膜印迹细胞学方法观察了春季结膜炎病程中结膜上皮的变化。对53例春季结膜炎患者和20例正常人进行了观察。印迹细胞学的结果表明，所有的细胞学参数显着修改春季结膜炎患者，最早的改变被发现在杯状细胞的分布，在细胞间连接，染色质形态和角化的程度。形态计量学比较显示，春季结膜炎患者的平均杯状细胞数显著高于对照组。此外，患者眼表杯状细胞平均直径小于对照组。因此，印模细胞学检查可作为一种简单、无创、廉价的方法用于研究春季结膜炎眼表。
The alterations in the conjunctival epithelium during the course of vernal conjunctivitis were examined by conjunctival impression cytology. The study was carried out on 53 patients,vith vernal conjunctivitis and 20 normal subjects as control. The results of impression cytology demonstrated that all cytological parameters were significantly modified in vernal conjunctivitis patients; the earliest alterations were found in the distribution of goblet cells, in the intercellular junctions, in the chromatin morphology and in the degree of keratinisation. The morphometric comparison showed that in vernal conjunctivitis patients the mean number of goblet cells per field was significantly higher than in controls. Moreover the mean diameter of goblet cells was smaller in patients than in controls, Impression cytology can, therefore, be a simple, non-invasive and cheap method for the study of the ocular surface in vernal conjunctivitis.