miR-330-3p suppresses liver cancer cell migration by targeting MAP2K1
miR-330-3p suppresses liver cancer cell migration by targeting MAP2K1
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DOI：
10.3892/ol.2019.10280
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发表时间：
2019-07-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Yahui
中科院分区：
文献类型：
--
作者：
Jin, Zhe;Jia, Baoxing;Liu, Yahui
MicroRNA 被认为是肿瘤治疗的一个有前景的焦点，是大量基因的关键调节因子。本研究的目的是研究 microRNA (miR)-330-3p 在肝癌中的生物学功能，因为之前已发现 miR-330-3p 在肝癌中失调。为了鉴定miR-330-3p在肝癌中的功能，采用逆转录定量聚合酶链反应分析测定肝癌组织和癌旁非肿瘤组织中miR-330-3p的表达。为了阐明 miR-330-3p 在肝癌中的功能，使用模拟转染过表达 miR-330-3p。在肝癌细胞中，miR-330-3p 抑制细胞迁移。 miRNA靶预测数据库用于鉴定肝癌中miR-330-3p的潜在靶基因。在肝癌细胞中，miR-330-3p 下调丝裂原激活蛋白激酶激酶 1 (MAP2K1) 的 RNA 水平。总之，miR-330-3p 通过靶向肝癌细胞中的 MAP2K1 来抑制细胞迁移。
MicroRNAs, considered as a promising focus for the treatment of tumors, are key regulators of a large number of genes. The aim of the present study was to investigate the biological functions of microRNA (miR)-330-3p in liver cancer as it had been identified previously that miR-330-3p was deregulated in liver cancer. In order to identify the function of miR-330-3p in liver cancer, the expression of miR-330-3p was determined in liver cancer tissues and adjacent non-tumor tissues using reverse transcription-quantitative polymerase chain reaction analysis. To elucidate the function of miR-330-3p in liver cancer, miR-330-3p was overexpressed using mimic transfection. Cell migration was inhibited by miR-330-3p in liver cancer cells. The miRNA target prediction databases were used to identify potential target genes of miR-330-3p in liver cancer. The RNA level of mitogen-activated protein kinase kinase 1 (MAP2K1) was downregulated by miR-330-3p in liver cancer cells. In conclusion, miR-330-3p suppresses cell migration by targeting MAP2K1 in liver cancer cells.