Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.
Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.
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DOI：
10.1007/978-1-4939-6892-3_17
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yang D
中科院分区：
文献类型：
--
作者：
Lin C;Yang D
G-四链体是在含有连续鸟嘌呤的DNA序列中形成的非规范二级结构。研究表明，人端粒中富含3′ G的单链突出端可以形成G-四链体结构，人端粒DNA G-四链体被认为是抗癌药物的理想靶点。G-四链体相互作用化合物已显示出抑制端粒酶接近以及端粒加帽。核磁共振（NMR）光谱是在生理相关条件下确定G-四链体结构的有力方法。我们提出的NMR和生物物理方法在我们的研究小组用于研究G-四链体结构在生理相关的解决方案和它们与小分子化合物的相互作用。
G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. It has been shown that the 3′ G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures, and the human telomeric DNA G-quadruplexes are considered attractive targets for anticancer drugs. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR and biophysical methodology used in our research group for the study of G-quadruplex structures in physiologically relevant solution and their interactions with small-molecule compounds.