TISSUE DISTRIBUTION OF S-ADENOSYLMETHIONINE AND S-ADENOSYLHOMOCYSTEINE IN RAT - EFFECT OF OF AGE, SEX AND METHIONINE ADMINISTRATION ON METABOLISM OF S-ADENOSYLMETHIONINE, S-ADENOSYLHOMOCYSTEINE AND POLYAMINES
TISSUE DISTRIBUTION OF S-ADENOSYLMETHIONINE AND S-ADENOSYLHOMOCYSTEINE IN RAT - EFFECT OF OF AGE, SEX AND METHIONINE ADMINISTRATION ON METABOLISM OF S-ADENOSYLMETHIONINE, S-ADENOSYLHOMOCYSTEINE AND POLYAMINES
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DOI：
10.1042/bj1660521
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发表时间：
1977-01-01
影响因子：
4.1
通讯作者：
ELORANTA, TO
中科院分区：
文献类型：
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作者：
ELORANTA, TO
研究了S-腺苷蛋氨酸、S-腺苷同型半胱氨酸、蛋氨酸腺苷转移酶和S-腺苷同型半胱氨酸水解酶在大鼠体内的组织分布。本文研究了蛋氨酸对大鼠肝、脑、肾组织中S-腺苷蛋氨酸、S-腺苷同型半胱氨酸和多胺蓄积的影响。S-腺苷蛋氨酸、S-腺苷同型半胱氨酸、蛋氨酸腺苷转移酶和S-腺苷同型半胱氨酸水解酶的组织分布在两性中相似，只是随着年龄的增加略有变化。S-腺苷同型半胱氨酸水解酶的比活力大大超过蛋氨酸腺苷转移酶，且所有组织中S-腺苷蛋氨酸的浓度高于S-腺苷同型半胱氨酸。肝脏S-腺苷蛋氨酸/S-腺苷-同型半胱氨酸的比值与食物供应和动物年龄有关。蛋氨酸腺苷转移酶活性与腺苷类化合物在不同组织中的浓度之间没有相关性。IP地址。给予蛋氨酸后，肝脏S-腺苷蛋氨酸和S-腺苷同型半胱氨酸浓度显著升高。注射蛋氨酸后2 h，脑组织中S-腺苷蛋氨酸的浓度也升高。同时给予甘氨酸可减轻蛋氨酸治疗后S-腺苷蛋氨酸浓度的升高。S-腺苷蛋氨酸合成的限速因素可能是组织中蛋氨酸的浓度。甘氨酸N-甲基转移酶可能对S-腺苷蛋氨酸在肝脏中的利用有调节作用。
Tissue distribution of S-adenosylmethionine, S-adenosylhomocysteine, methionine adenosyltransferase and S-adenosylhomocysteine hydrolase was explored in rats. The effects of methionine administration on accumulation of S-adenosylmethionine, S-adenosylhomocysteine and polyamines were studied in rat liver, brain and kidney. Tissue distribution of S-adenosylmethionine, S-adenosylhomocysteine, methionine adenosyltransferase and S-adenosylhomocysteine hydrolase was similar in both sexes, and only slightly changed with age. Specific activity of S-adenosylhomocysteine hydrolase greatly exceeded that of methionine adenosyltransferase, and the concentration of S-adenosylmethionine was higher than S-adenosylhomocysteine in all tissues examined. The hepatic S-adenosylmethionine/S-adenosylhomocysteine ratio was dependent on food supply and animal age. No correlation was noticed between the activity of methionine adenosyltransferase and concentrations of the adenosyl compounds in different tissues. I.p. administration of methionine resulted in a profound but transient increase in hepatic concentrations of S-adenosylmethionine and S-adenosylhomocysteine. The concentration of S-adenosylmethionine was elevated also in the brain during the first 2 h after methionine injection. The rise of S-adenosylmethionine concentration after methionine treatment could be diminished by simultaneous glycine administration. The rate-limiting factor of S-adenosylmethionine synthesis is probably the tissue concentration of methionine. Glycine N-methyltransferase may have a regulatory role in utilization of S-adenosylmethionine in the liver.