Serotonin Transport by Cultured Bovine Aortic Endothelium
Serotonin Transport by Cultured Bovine Aortic Endothelium
复制标题
培养牛主动脉内皮的血清素转运
DOI：
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发表时间：
1975
影响因子：
20.1
通讯作者：
F. A. Belamarich
中科院分区：
文献类型：
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作者：
D. Shepro;J. Batbouta;L. Robblee;M. P. Carson;F. A. Belamarich
Endothelial cells isolated from bovine aortas without prior treatment with enzymes were cultured in RPMI 1640 medium containing 17% fetal calf serum and antibiotics. The endothelial cells at confluency (7 days) were similar to endothelium in situ or to freshly isolated endothelial cells from blood vessels as seen by light, scanning, and electron microscopy. Cultured and freshly isolated endothelial cells exposed to labeled serotonin, even in the presence of iproniazid (5 × 10−4m), took up approximately 125 and 250 pmoles 14C-serotonin/mg protein, respectively, in 3 hours. Imipramine (10−4m) reduced uptake for both cell groups. Cold (4°C) and metabolic inhibitors sharply reduced serotonin uptake by both freshly isolated and cultured endothelial cells. Ouabain (10−5m) almost completely blocked serotonin transport. Six analogues of serotonin at concentrations ten times above experimental serotonin concentrations did not affect serotonin transport in the cultured endothelial cells but did reduce it in the freshly isolated endothelial cells by 50%. The data on transport suggest that serotonin uptake is not unique to pulmonary endothelium, as has been suggested previously. In addition, using cultured endothelial cells to study serotonin transport is compatible with using other serotonin model systems such as platelets, lung, or brain. Lastly, serotonin uptake by endothelial cells may involve an active transport mechanism similar to that described for the pulmonary circulation, platelets, and insect salivary glands.
