Temporal kinetics of CD8(+)CD28(+) and CD8(+)CD28(-) T lymphocytes in the injured rat spinal cord
Temporal kinetics of CD8(+)CD28(+) and CD8(+)CD28(-) T lymphocytes in the injured rat spinal cord
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损伤大鼠脊髓中CD8( )CD28( )和CD8( )CD28(-) T淋巴细胞的时间动力学
DOI：
10.1002/jnr.23993
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发表时间：
2017
影响因子：
4.2
通讯作者：
Lü HZ
中科院分区：
文献类型：
--
作者：
Wu Y;Lin YH;Shi LL;Yao ZF;Xie XM;Jiang ZS;Tang J;Hu JG;Lü HZ
本研究旨在通过免疫组织化学（IHC）和流式细胞术（FCM）相结合的方法，探讨大鼠脊髓损伤（SCI）后损伤微环境中细胞毒性CD 8 + CD 28+和调节性CD 8 + CD 28 −T细胞亚群的时间变化。脊髓损伤后，CD 8 + T细胞在伤后第1天即开始出现，随后明显增加，伤后3、7、14天明显高于伤后1天（p < 0.01），伤后7天最高。在CD 8 +T细胞中，90%以上是CD 28+，只有一小部分是CD 28-（< 10%）。14 d后，浸润的CD 8 +T细胞明显减少，21 d和28 d时几乎没有状态良好的CD 8 + T细胞。Annexin V和碘化丙啶（PI）染色显示，14 dpi和21 dpi时凋亡/坏死CD 8+细胞的百分比显著高于其他早期时间点（p < 0.01）。这些结果表明，CD 8 +T细胞可以迅速浸润到损伤的脊髓中并存活两周，但细胞毒性CD 8 +T细胞占主导地位。因此，损伤后两周可能是通过延长生存时间和增加CD 8+调节性T细胞比例来治疗SCI的“时间窗”。© 2016 Wiley Periodicals，Inc.
This study aims to explore the temporal changes of cytotoxic CD8+CD28+and regulatory CD8+CD28−T‐cell subsets in the lesion microenvironment after spinal cord injury (SCI) in rats, by combination of immunohistochemistry (IHC) and flow cytometry (FCM). In the sham‐opened spinal cord, few CD8+T cells were found. After SCI, the CD8+T cells were detected at one day post‐injury (dpi), then markedly increased and were significantly higher at 3, 7, and 14 dpi compared with one dpi (p < 0.01), the highest being seven dpi. In CD8+T cells, more than 90% were CD28+, and there were only small part of CD28−( < 10%). After 14 days, the infiltrated CD8+T cells were significantly decreased, and few could be found in good condition at 21 and 28 dpi. Annexin V and propidium iodide (PI) staining showed that the percentages of apoptotic/necrotic CD8+cells at 14 dpi and 21 dpi were significantly higher than those of the other early time‐points (p < 0.01). These results indicate that CD8+T cells could rapidly infiltrate into the injured spinal cords and survive two weeks, however, cytotoxic CD8+T cells were dominant. Therefore, two weeks after injury might be the “time window” for treating SCI by prolonging survival times and increasing the fraction of CD8+regulatory T‐cells. © 2016 Wiley Periodicals, Inc.