Egg yolk-free Baird-Parker medium for the accelerated enumeration of foodborne Staphylococcus aureus

Egg yolk-free Baird-Parker medium for the accelerated enumeration of foodborne Staphylococcus aureus
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用于加速计数食源性金黄色葡萄球菌的无蛋黄 Baird-Parker 培养基

DOI:
10.1128/aem.48.4.870-871.1984
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发表时间:
1984
影响因子:
4.4
通讯作者:
R. Lachica
R. Lachica
中科院分区:
生物学2区
文献类型:
--
作者:
R. Lachica

文献摘要

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介绍了一种用于食源性金黄色葡萄球菌加速计数的简化程序。这涉及用吐温80和MgCl 2替换Baird-Parker培养基中的蛋黄。这些化合物,沿着丙酮酸盐,使应激的S.金黄色葡萄球菌在含有亚碲酸钾、LiCl和甘氨酸作为选择剂的培养基上培养。黑色菌落被鉴定为S。金黄色葡萄球菌的简化热核酸酶试验。
A simplified procedure is described for the accelerated enumeration of foodborne Staphylococcus aureus. This involves the replacement of egg yolk in the Baird-Parker medium with Tween 80 and MgCl2. These compounds, along with pyruvate, allow the recovery of stressed cells of S. aureus on a medium which contains potassium tellurite, LiCl, and glycine as selective agents. Black colonies are identified as S. aureus by the simplified thermonuclease test.