A rabbit model of hormone-induced early avascular necrosis of the femoral head.
A rabbit model of hormone-induced early avascular necrosis of the femoral head.
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DOI：
10.1016/s0895-3988(08)60060-4
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Wang XN
中科院分区：
文献类型：
--
作者：
Wen Q;Ma L;Chen YP;Yang L;Luo W;Wang XN
目的建立糖皮质激素致兔早期股骨头缺血性坏死(ANFH)动物模型，并应用多种影像技术观察其病理变化。ANFH是由注射马血清引起的过敏性脉管炎和随后给予高剂量皮质类固醇引起的组合诱发的。采用数字化X线摄影(DR)、计算机体层摄影(CT)、磁共振成像(MRI)、墨汁动脉灌注血管造影术、苏木精-伊红染色和免疫组织化学等方法检测病理改变。影像和病理改变符合早期股骨头坏死的临床特点。DR显示双侧骨密度增加，骨痂轮廓不清，松质骨质地模糊。CT表现为松质骨斑点状低密度影，皮质变薄，骨质疏松，骨骺线不清。MRI表现为骨髓水肿，T2加权像松质骨呈点状高信号。墨汁动脉灌注血管造影术显示股骨头血管阻塞较少。病理切片HE染色可见骨小梁减少，骨质变薄，空骨陷窝比例增加，造血减少，血栓形成，脂肪细胞肥大。免疫组织化学显示血管内皮生长因子在成骨细胞和软骨细胞以及血管内膜上的表达减弱。本实验成功建立了糖皮质激素所致早期股骨头坏死动物模型，为开发治疗早期股骨头坏死的有效方法奠定了基础。
To establish an experimental model of early stage avascular necrosis of the femoral head (ANFH) caused by corticosteroid in adult rabbits and to observe the pathological changes with various imaging techniques. ANFH was induced by a combination of hypersensitivity vasculitis caused by injection of horse serum and subsequent administration of a high dose of corticosteroid. The pathological changes were detected with digital radiography (DR), computed tomography (CT), magnetic resonance imaging (MRI), ink artery infusion angiography, hematoxylin–eosin staining, and immunohistochemistry. The imageological and pathological changes corresponded to the clinical characteristics of early stage ANFH. DR showed bilaterally increased bone density, an unclear epiphyseal line, and blurred texture of cancellous bone. CT showed spot-like low-density imaging of cancellous bone, thinner cortical bone, osteoporosis, and an unclear epiphyseal line. MRI showed bone marrow edema and spot-like high signals in T2-weighted imaging in cancellous bone. Ink artery infusion angiography showed fewer obstructed blood vessels in the femoral head. HE staining of pathological sections showed fewer trabeculae and thin bone, an increased proportion of empty osteocyte lacunae, decreased hematopoiesis, thrombosis, and fat cell hypertrophy. Immunohistochemistry showed attenuated expression of vascular endothelial growth factor in osteoblasts and chondrocytes, and on the inner membrane of blood vessels. Experimental rabbit model of early stage ANFH caused by corticosteroid can be successfully established and provide the foundation for developing effective methods to treat early stage ANFH.