Protein-poly(amino acid) complex precipitation for high-concentration protein formulation.
Protein-poly(amino acid) complex precipitation for high-concentration protein formulation.
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用于高浓度蛋白质配方的蛋白质-聚（氨基酸）复合物沉淀。
DOI：
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Shiraki
中科院分区：
文献类型：
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作者：
Takaaki Kurinomaru;T. Maruyama;S. Izaki;K. Handa;Tomoaki Kimoto;K. Shiraki
高剂量蛋白质制剂需要一种浓缩蛋白质溶液的方法。在这里，我们提出了一种沉淀-复溶方法聚（氨基酸）的蛋白质，包括治疗酶，抗体和激素。通过在低离子强度下加入聚-L-赖氨酸或聚-L-谷氨酸使蛋白质完全沉淀，之后在生理离子强度下溶解沉淀物。再溶解的蛋白质，特别是抗体的活性和二级结构几乎与天然状态相同。沉淀-再溶解法是一种简单、快速的蛋白质制剂浓缩技术。
A method for concentration of protein solutions is required for high-dosage protein formulation. Here, we present a precipitation-redissolution method by poly(amino acid) for proteins, including therapeutic enzymes, antibodies, and hormones. The proteins were fully precipitated by the addition of poly-L-lysine or poly-L-glutamic acid at low ionic strength, after which precipitate was dissolved at physiological ionic strength. The activities and secondary structures of redissolved proteins, especially antibodies, were almost identical to the native state. The precipitation-redissolution method is a simple and rapid technique for concentration of protein formulations.
影响因子：
2.9
作者：
ARAKAWA, T;TIMASHEFF, SN
通讯作者：
TIMASHEFF, SN
DOI：
10.1016/s0021-9258(18)43240-1
发表时间：
1981-12
期刊：
The Journal of biological chemistry
影响因子：
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作者：
D. Atha;K. Ingham
通讯作者：
D. Atha;K. Ingham