Prevalence and Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolated from Cattle between 2002 and 2006 in France
Prevalence and Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolated from Cattle between 2002 and 2006 in France
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DOI：
10.4315/0362-028x-73.5.825
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发表时间：
2010-05-01
影响因子：
2
通讯作者：
Madec, Jean-Yves
中科院分区：
文献类型：
--
作者：
Chatre, Pierre;Haenni, Marisa;Madec, Jean-Yves
来自法国九个部门的2255头牛(小牛、小肉牛和被扑杀的奶牛)的粪便在屠宰时被收集。在2,255个样本中，16.5%的样本(空肠弯曲菌12.8%，大肠埃希菌3.7%)中发现了弯曲杆菌，主要是从小牛身上提取的。用E-test检测对6种医用和/或兽医用抗生素的耐药性。大多数菌株对四环素耐药(52.8%的空肠肠杆菌和88.1%的大肠埃希菌)，而对氨苄西林和红霉素的耐药率较低，但仍保持一致。只有两株大肠埃希菌对庆大霉素耐药。空肠弯曲菌和大肠埃希菌均存在多重耐药，其中0.8%(3/372)的菌株对6种抗菌药物中的5种耐药。空肠弯曲菌对氟喹诺酮类和氟喹诺酮类药物的耐药率呈上升趋势，2006年奈利地酸耐药率达70.4%，2002~2006年间耐氟喹诺酮类药物由29.7%上升至70.4%。所有数据都使用临床断点或流行病学截止值进行了平行分析，结果大部分重叠，庆大霉素除外。这项为期5年的调查(2002年至2006年)首次概述了空肠弯曲菌和大肠埃希氏菌在法国牛中的流行情况和抗药性，并在关于人类弯曲杆菌与牲畜之间联系的经常性报告的背景下，记录了牛对法国弯曲杆菌环境宿主的贡献程度。这一结果表明，牛空肠弯曲菌对氟喹诺酮类药物的抗药性显著增加，这可能对公共卫生具有重要意义。
Feces from 2,255 cattle (calves, young beef cattle, and culled cows) were collected at slaughter from nine departments across France. Campylobacter was recovered from 16.5% of the 2,255 samples (C. jejuni from 12.8% and C. coli from 3.7%), predominantly from calves. Antimicrobial resistance to six antibiotics of medical and/or veterinary interest was tested with the E-test. Resistance to tetracycline was found in most isolates (52.8% of C. jejuni isolates and 88.1% of C. coli isolates) in contrast to low but consistent resistance to ampicillin and erythromycin. Only two C. coli isolates were resistant to gentamicin. Multiple resistance was frequently detected in C. jejuni and C. coli isolates, and 0.8% (3 of 372) of the isolates were resistant to five of the six antimicrobials. An upward trend in the resistance to quinolones and fluoroquinolones in C. jejuni from calves was found; resistance to nalidixic acid reached 70.4% in 2006 and fluoroquinolone resistance increased from 29.7 to 70.4% during 2002 through 2006. All data were analyzed in parallel using clinical breakpoints or epidemiological cutoff values, and the results overlapped largely, except those for gentamicin. This 5-year survey (2002 through 2006) gives the first overview of the prevalence and antimicrobial resistance of C. jejuni and C. coli in cattle in France and documents to what extent cattle may contribute to the environmental reservoir of Campylobacter in France in the context of recurrent reports on links between human campylobacterioses and livestock. The results underline a notable increase in the resistance to fluoroquinolones in C. jejuni from cattle that may be of significant importance for public health.