Differences in mitotic control among mammalian cells.
Differences in mitotic control among mammalian cells.
复制标题
哺乳动物细胞之间有丝分裂控制的差异。
DOI：
10.1101/sqb.1991.056.01.049
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Sherwood,SW
中科院分区：
文献类型：
--
作者：
Schimke,RT;Kung,AL;Rush,DF;Sherwood,SW
材料和方法用流式细胞术分析细胞系、它们的生长和细胞周期进展的方法已在之前描述过（Kung et al. 1990a）。先前也描述了放射治疗方案（Sharma和Schimke 1989）。放射后2-4小时加入细胞松弛素D （Sigma）至终浓度2/xg/ml。使用细胞学软件包（Coulter Electronics）进行流式细胞术细胞周期分析，每个细胞周期阶段的细胞频率代表五种不同DNA分析模型的平均值。细胞大小通过前角光散射（FALS）测量，FALS与固定细胞的总细胞蛋白含量密切相关（Sherwood et al. 1988）。所有采用细胞同步的实验都使用了有丝分裂“震荡”技术（Kung et al. 1990a），因为各种化学和物理同步技术可能导致细胞周期的正常进程受到干扰。实验的其他细节在图的图例中有描述。
MATERIALS AND METHODSThe cell lines, their growth, and cell cycle progression analysis by flow cytometric methods were described previously (Kung et al. 1990a). y radiation treatment protocols were also described previously (Sharma and Schimke 1989). Cytochalasin D (Sigma) was added 2-4 hours postirradiation to a final concentration of 2/xg/ml. Cell cycle analysis by flow cytometry was performed with the Cytologic Software package (Coulter Electronics), and cell frequency in each cell cycle phase represents the average value derived from five different DNA analysis models. Cell size was measured as forward angle light scatter (FALS), which correlates well with total cell protein content in fixed cells (Sherwood et al. 1988). All experiments employing cell synchronization used mitotic" shake-off" techniques (Kung et al. 1990a), inasmuch as various chemical and physical synchronization techniques may result in perturbation of the normal progression of the cell cycle. Other specifics of the experiments are described in the legends to the figures.