Characteristics of an Extended Gate Field-Effect Transistor for Glucose Sensing Using an Enzyme-Containing Silk Fibroin Membrane as the Bio-Chemical Component
Characteristics of an Extended Gate Field-Effect Transistor for Glucose Sensing Using an Enzyme-Containing Silk Fibroin Membrane as the Bio-Chemical Component
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DOI：
10.3390/bios10060057
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
K. Koike;Taihou Sasaki;Kenta Hiraki;K. Ike;Y. Hirofuji;M. Yano
中科院分区：
文献类型：
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作者：
K. Koike;Taihou Sasaki;Kenta Hiraki;K. Ike;Y. Hirofuji;M. Yano
报道了一种基于离子敏感型TiO 2/Ti扩展栅场效应晶体管（EGFET）的葡萄糖传感器的特性。将含有葡萄糖氧化酶的丝素蛋白膜固定在TiO 2/Ti表面作为生物传感元件。这种EGFET型生物传感器估计能够检测水性电解质中低至0.001 mg/mL的葡萄糖浓度，这使得能够感测唾液和汗液中的葡萄糖。还考察了这种传感器的耐久性，发现在室温下重复使用的原始灵敏度保持时间超过30天，具有接近60 ℃的高耐热温度。
The characteristics of a glucose sensor based on an ion-sensitive TiO2/Ti extended gate electrode field-effect transistor (EGFET) are reported. A glucose oxidase-containing silk fibroin membrane was immobilized on a TiO2/Ti surface as the bio-sensing component. This EGFET-type biosensor was estimated to be able to detect a glucose concentration as low as 0.001 mg/mL in an aqueous electrolyte, which enables the sensing of glucose in the saliva and sweat. The endurance of this sensor was also examined, and it was found that the retention time of the original sensitivity for repeated use at room temperature was more than 30 days, with a high heat tolerance temperature close to 60 °C.