Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies
Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies
复制标题
DOI：
10.1016/s1090-7807(03)00273-8
复制
发表时间：
2003-11-01
影响因子：
2.2
通讯作者：
Wider, G
中科院分区：
文献类型：
--
作者：
Braun, D;Wüthrich, K;Wider, G
应用于生物大分子溶液的现代核磁共振实验通常包括多个磁化转移步骤。当处理大型结构时，在这些转移过程中会损失很大一部分磁化。对于复杂实验方案的设计和优化，磁化转移效率通常由自旋弛豫时间计算。本文提出了一种新的方法，用短期可靠的实验直接测量个人转移效率。该方法在110,000 Da蛋白上的初步应用表明，在这种大小的结构中，单个自旋对之间的转移效率范围很广，这导致了相应的单个信号强度的大变化，并且需要技术来增强弱信号。(C) 2003 Elsevier Inc.版权所有。
Modern NMR experiments for applications with biological macromolecules in solution typically include multiple magnetization transfer steps. When working with large structures, a significant fraction of the magnetization is lost during these transfers. For the design and optimization of complex experimental schemes, the magnetization transfer efficiencies have therefore commonly been calculated from the spin relaxation times. This paper now suggests a new method for measurement of individual transfer efficiencies directly with the system of interest, using short, reliable experiments. Initial applications of this approach with a 110,000 Da protein indicate that there is a wide range of transfer efficiencies among individual spin pairs in a structure of this size, which leads to a correspondingly large variation of the individual signal intensities and the need for techniques to enhance the weak signals. (C) 2003 Elsevier Inc. All rights reserved.