Bone grafts cultured with bone marrow stromal cells for the repair of critical bone defects: An experimental study in mice
Bone grafts cultured with bone marrow stromal cells for the repair of critical bone defects: An experimental study in mice
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DOI：
10.1002/jbm.a.32176
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发表时间：
2009-09-15
影响因子：
4.9
通讯作者：
Chappard, Daniel
中科院分区：
文献类型：
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作者：
Dumas, Aline;Moreau, Marie-Francoise;Chappard, Daniel
自体骨组织工程复合骨祖细胞是一种较好的修复大块骨缺损的方法。本研究的目的是评价异种骨移植与同种异体骨髓基质细胞(BMSC)在小鼠临界大小开颅手术中的成骨活性。牛骨小梁移植物去除骨髓细胞或碎屑，并进行脱脂处理。从C57BL/6-TG(ACTbEGFP)1Osb/J小鼠(GFP(+)细胞)中获取骨髓间充质干细胞，在对照组和成骨培养基骨移植上培养14天。将基因工程骨移植到C57BL/6小鼠的颅骨缺损处。实验分为4组：骨髓间充质干细胞成骨分化移植组(G-Ob)、骨髓间充质干细胞移植组(G-BMSC)、无细胞移植组(G)和未移植组。分别于植入后2周和8周取颅盖骨进行X线片和组织形态计量学分析。G组：骨长入局限于缺损区边缘。移植物的中心由纤维血管结缔组织填充。G-BMSC或G-Ob组：缺损区中心早期成骨，2~8周未见增加，新形成的编织骨部分被板层骨替代。术前骨髓间充质干细胞向成骨细胞分化不利于更快更好的骨再生。2周后，移植骨周围可见GFP(+)细胞，而新生骨中未见GFP(+)骨细胞。8周后未见GFP(+)细胞。然而，植入前培养的生物材料与同种异体骨髓间充质干细胞大大促进了骨再生。(C)2008 Wiley期刊，Inc.《生物医学杂志》90A：1218-1229,2009
Tissue engineering of autologous bone combined with osteoprogenitor cells is a suitable strategy for filling large bone defects. The aim of this study was to evaluate the osteogenicity of a xenogenic bone graft cultured with allogenic bone marrow stromal cells (BMSC) in a mouse critical size craniotomy. Bovine trabecular bone grafts were made free of bone marrow cells or debris and were delipidated. BMSC were harvested from C57BL/6-Tg(ACTbEGFP)1Osb/J mice (GFP(+) cells) and were cultured 14 days on bone grafts in control or osteogenic medium. Engineered grafts were implanted in calvarial defect in C57BL/6 mice. Four groups were studied: graft with BMSC differentiated in osteoblasts (G-Ob), graft with BMSC (G-BMSC), graft without cells (G) and no graft. Calvariae were studied 2 and 8 weeks after implantation by radiographic and histomorphometric analyses. G group: the bone ingrowth was limited to the edges of the defect. The center of the graft was filled by a fibrovascular connective tissue. G-BMSC or G-Ob groups: bone formation occurred early in the center of the defect and did not increase between 2 and 8 weeks; the newly formed woven bone was partially replaced by lamellar bone. The preoperative osteoblastic differentiation of BMSC did not allow faster and better bone regeneration. After 2 weeks, GFP(+) cells were observed around the grafted bone but no GFP(+) osteocyte was present in the newly formed bone. No GFP(+) cell was noted after 8 weeks. However, pre-implantation culture of the biomaterial with allogenic BMSC greatly enhanced the bone regeneration. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 1218-1229, 2009