Tissue Equivalents Based on Cell-Seeded Biodegradable Microfluidic Constructs
Tissue Equivalents Based on Cell-Seeded Biodegradable Microfluidic Constructs
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DOI：
10.3390/ma3031833
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发表时间：
2010-03-15
期刊：
影响因子：
3.4
通讯作者：
Herman IM
中科院分区：
文献类型：
--
作者：
Borenstein JT;Megley K;Wall K;Pritchard EM;Truong D;Kaplan DL;Tao SL;Herman IM
组织工程和再生医学领域的主要挑战之一是形成能够维持组织构建体的功能性微血管网络。复杂的组织和重要器官需要在宿主整合之前和之后的构建体开发期间支持氧气和营养物运输的手段，并且目前的方法尚未证明针对该挑战的稳健解决方案。在这里，我们提出了一个技术平台，包括伤口愈合和其他临床应用的组织等效物的设计，构建，细胞接种和功能评价。这些组织等同物由内衬有微血管细胞的可生物降解的微流体支架组成，并被设计为复制产生和维持细胞群所必需的微环境线索，以替代由于创伤或疾病而丢失的真皮和/或表皮组织。初步结果表明，这些可生物降解的微流体装置促进细胞粘附并支持基本的细胞功能。这些系统代表了一个有前途的途径，高度集成的三维工程组织构建广泛的临床应用。
One of the principal challenges in the field of tissue engineering and regenerative medicine is the formation of functional microvascular networks capable of sustaining tissue constructs. Complex tissues and vital organs require a means to support oxygen and nutrient transport during the development of constructs both prior to and after host integration, and current approaches have not demonstrated robust solutions to this challenge. Here, we present a technology platform encompassing the design, construction, cell seeding and functional evaluation of tissue equivalents for wound healing and other clinical applications. These tissue equivalents are comprised of biodegradable microfluidic scaffolds lined with microvascular cells and designed to replicate microenvironmental cues necessary to generate and sustain cell populations to replace dermal and/or epidermal tissues lost due to trauma or disease. Initial results demonstrate that these biodegradable microfluidic devices promote cell adherence and support basic cell functions. These systems represent a promising pathway towards highly integrated three-dimensional engineered tissue constructs for a wide range of clinical applications.