Transcriptomic analysis of resistant and susceptible responses in a new model root-knot nematode infection system using Solanum torvum and Meloidogyne arenaria
Transcriptomic analysis of resistant and susceptible responses in a new model root-knot nematode infection system using Solanum torvum and Meloidogyne arenaria
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使用托鲁巴姆和根结线虫对新型根结线虫感染系统中的抗性和易感反应进行转录组分析
DOI：
10.1101/2021.04.02.438176
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发表时间：
2021
影响因子：
5.6
通讯作者：
S
中科院分区：
文献类型：
--
作者：
Sato Kazuki;Uehara Taketo;Holbein Julia;Sasaki-Sekimoto Yuko;Gan Pamela;Bino Takahiro;Yamaguchi Katsushi;Ichihashi Yasunori;Maki Noriko;Shigenobu Shuji;Ohta Hiroyuki;Franke Rochus B.;Siddique Shahid;Grundler Florian M. W.;Suzuki Takamasa;Kadota Yasuhiro;S
根结线虫（Root-knot nematodes，RKN）是农业上最具破坏性的害虫之一。土耳其莓（Turkey berry）对南方根结线虫（Meloidogyne incognita）和M.砂壳我们以前发现M. arenaria，A2-J能侵染并繁殖。体外感染试验表明，S. Torvum在无毒致病型A2-O感染时诱导褐色素积累，而在毒力A2-J感染时不诱导褐色素积累。该实验系统是有利的，因为可以在几天内区分抗性和敏感反应，并且因为单个植物基因组可以产生关于抗性和敏感反应的信息。S.用A2-J和A2-O在侵染初期接种番茄，分析其特异性抗性和敏感性反应。A2-J感染后一天内基因表达没有显著变化，但随后，A2-J特异性地诱导查尔酮合酶、亚精胺合酶以及与细胞壁修饰和跨膜转运相关的基因的表达。感染A2-O迅速诱导表达的基因编码的III类过氧化物酶，倍半萜烯脱氢酶，脂肪酸脱氢酶在1 DPI，其次是基因参与防御，激素信号，和生物合成的木质素在3 DPI。这两个菌株诱导木栓质生物合成基因的表达，这可能是由线虫感染过程中的伤口触发。组织化学分析表明，A2-O，而不是A2-J，诱导木质素积累在根尖，这表明，物理加固细胞壁与木质素是一个重要的防御反应对线虫。帖前torvum-RKN系统的建立为研究植物-线虫相互作用提供了分子基础。
Root-knot nematodes (RKNs) are among the most devastating pests in agriculture.Solanum torvumSw. (Turkey berry) has been used as a rootstock for eggplant (aubergine) cultivation because of its resistance to RKNs, includingMeloidogyne incognitaandM. arenaria. We previously found that a pathotype ofM. arenaria, A2-J, is able to infect and propagate inS. torvum. In vitroinfection assays showed thatS. torvuminduced the accumulation of brown pigments during avirulent pathotype A2-O infection, but not during virulent A2-J infection. This experimental system is advantageous because resistant and susceptible responses can be distinguished within a few days, and because a single plant genome can yield information about both resistant and susceptible responses. Comparative RNA-sequencing analysis ofS. torvuminoculated with A2-J and A2-O at early stages of infection was used to parse the specific resistance and susceptible responses. Infection with A2-J did not induce statistically significant changes in gene expression within one day post-inoculation (DPI), but afterward, A2-J specifically induced the expression of chalcone synthase, spermidine synthase, and genes related to cell wall modification and transmembrane transport. Infection with A2-O rapidly induced the expression of genes encoding class III peroxidases, sesquiterpene synthases, and fatty acid desaturases at 1 DPI, followed by genes involved in defense, hormone signaling, and the biosynthesis of lignin at 3 DPI. Both isolates induced the expression of suberin biosynthetic genes, which may be triggered by wounding during nematode infection. Histochemical analysis revealed that A2-O, but not A2-J, induced lignin accumulation at the root tip, suggesting that physical reinforcement of cell walls with lignin is an important defense response against nematodes. TheS. torvum-RKN system can provide a molecular basis for understanding plant-nematode interactions.