Transcriptome sequencing identified wax-related genes controlling the glossy phenotype formation of "Ganqi 3," a bud mutant derived from wild-type "Newhall" navel orange
Transcriptome sequencing identified wax-related genes controlling the glossy phenotype formation of "Ganqi 3," a bud mutant derived from wild-type "Newhall" navel orange
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转录组测序鉴定出控制“甘其 3”光泽表型形成的蜡相关基因，“甘其 3”是一种源自野生型“Newhall”脐橙的芽突变体
DOI：
10.1007/s11295-016-1017-8
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发表时间：
2016-06-01
影响因子：
2.4
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Liu, Dechun;Yang, Li;Liu, Yong
植物气生表面覆盖着表皮蜡，这是一种保护植物器官免受生物和非生物胁迫的屏障结构。虽然脐橙橙子（Citrus sinensis [L.] Osbeck）的研究，但蜡质生物合成和输出的分子机制尚不清楚。本研究以纽荷尔脐橙橙子（WT）及其光滑突变体“甘齐3号”（MT）为材料，构建了两个果皮cDNA文库，并利用Illumina测序平台进行测序，以鉴定蜡合成和输出相关的差异表达基因（DEG）。从WT和MT水果中总共鉴定出72种蜡相关DEG。其中48个DEG编码功能蛋白，3个DEG编码转录因子，4个DEG参与转录后过程，2个DEG参与表皮蜡质合成，15个DEG编码ABCG转运蛋白。大多数蜡质相关DEG，包括52个功能DEG、3个编码TF的DEG、3个转录后DEG和2个翻译后DEG，在MT果实中的表达水平降低，表明橙子表皮蜡质生物合成在转录、转录后和翻译后水平受到调控。此外，许多DEG与胁迫反应、激素介导的信号通路和发育过程有关，表明脐橙橙子表皮蜡质的生物合成受到各种胁迫、激素水平和发育过程的影响。结合我们以前研究WT和MT果实之间蜡质结构和化学组成差异的结果，我们认为MT果实果皮中大多数蜡质相关DEG表达的减少导致MT果实表面脂肪族蜡质的减少，导致表皮晶体数量减少，最终导致MT果实表面有光泽。
Plant aerial surfaces are covered with cuticular waxes, a barrier structure protecting plant organs from biotic and abiotic stresses. Although the wax ultrastructure and composition in navel orange (Citrus sinensis [L.] Osbeck) have been studied in various reports, the molecular mechanisms of wax biosynthesis and export are still unknown. In this report, two cDNA libraries from the fruit peels of "Newhall" navel orange (WT) and its glossy mutant "Ganqi 3" (MT) were constructed and sequenced using the Illumina sequencing platform to identify differentially expressed genes (DEGs) involved in wax biosynthesis and export. A total of 72 wax-related DEGs were identified from WT and MT fruits. Of these, 48 DEGs encoded functional proteins, 3 DEGs encoded transcription factors (TFs), 4 DEGs were involved in post-transcriptional processes, 2 DEGs with post-translational functions were involved in cuticular wax biosynthesis, and 15 DEGs encoding ABCG transporters were related to wax transport. Most wax-related DEGs, including 52 functional DEGs, 3 DEGs encoding TFs, 3 post-transcriptional DEGs, and 2 post-translational DEGs exhibited decreased expression levels in MT fruits, suggesting that cuticular wax biosynthesis in navel orange was regulated at the transcriptional, post-transcriptional, and post-translational levels. Moreover, many DEGs were related to the stress response, hormone-mediated signaling pathways, and developmental processes, suggesting that cuticular wax biosynthesis in navel orange was affected by various stresses, hormone levels, and developmental processes. Combined with the results from our previous studies investigating the wax structure and chemical composition differences between WT and MT fruits, we suggest that the decrease in most wax-related DEG expressions in MT fruit peels resulted in the decrease of aliphatic wax on MT fruit surfaces, leading to a reduced number of epicuticular crystals and finally resulting in the glossy surface of MT fruits.