Antiallergic Asthma Properties of Brazilin through Inhibition of TH2 Responses in T Cells and in a Murine Model of Asthma
Antiallergic Asthma Properties of Brazilin through Inhibition of TH2 Responses in T Cells and in a Murine Model of Asthma
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DOI：
10.1021/jf2038778
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发表时间：
2012-09-19
影响因子：
6.1
通讯作者：
Lai, Yu-Ting
中科院分区：
文献类型：
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作者：
Lee, Chen-Chen;Wang, Chien-Neng;Lai, Yu-Ting
本研究旨在确定巴西木素是否具有抑制 II 型辅助 T 细胞 (T(H)2) 反应的抗炎作用，以及它是否可以抑制小鼠哮喘模型中的过敏性炎症反应。我们发现巴西林以剂量依赖性方式抑制佛波醇肉豆蔻酸酯乙酸酯（PMA）和cAMP诱导的EL-4 T细胞中白细胞介素（IL）-4和IL-5的mRNA和蛋白表达。在卵清蛋白（OVA）免疫小鼠气管内滴注巴西林后，我们发现巴西林处理的小鼠支气管肺泡灌洗液（BALF）中IL-4、IL-5、IL-13、eotaxin-1和肿瘤坏死因子-α的释放减少；通过减少 IL-4 的产生来抑制 T(H)2 功能；并表现出 OVA 诱导的肺嗜酸性粒细胞增多、气道高反应性和气道重塑的减弱。这些结果表明巴西林在体外和体内均表现出抗 T(H)2 作用，并且可能具有治疗过敏性疾病的潜力。
This study aimed to determine whether brazilin exhibits anti-inflammatory effects that inhibit T helper cell type II (T(H)2) responses and whether it suppresses allergic inflammation reactions in a murine model of asthma. We found that brazilin inhibited the mRNA and protein expression of interleukin (IL)-4 and IL-5 induced by phorbol myristate acetate (PMA) and cAMP in EL-4 T cells in a dose-dependent manner. Following the intratracheal instillation of brazilin in ovalbumin (OVA)immunized mice, we found that brazilin-treated mice exhibited decreases in the release of IL-4, IL-5, IL-13, eotaxin-1, and tumor necrosis factor-alpha in bronchoalveolar lavage fluid (BALF); inhibited T(H)2 functioning via a decrease in IL-4 production; and exhibited attenuation of OVA-induced lung eosinophilia, airway hyperresponsiveness, and airway remodeling. These results suggest that brazilin exhibits anti-T(H)2 effects both in vitro and in vivo and may possess therapeutic potential for allergic diseases.