Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon
Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon
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DOI：
10.1677/joe.1.06494
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发表时间：
2006-03-01
影响因子：
4
通讯作者：
Tsutsui, K
中科院分区：
文献类型：
--
作者：
Amano, M;Moriyama, S;Tsutsui, K
我们最近确定了一个cDNA编码三种新的鱼类下丘脑神经肽，具有LPXRF-NH 2从金鱼的大脑。在这项研究中，为了阐明这三种LPXRFamide肽（gfLPXRFa-1，-2和-3）的生理功能，我们分析了这些肽的定位和垂体营养活性使用红鲑鱼，Oncorhynchus nerka，其中几个内部垂体激素的免疫测定系统已经开发。gfLPXKFa免疫反应阳性细胞体在下丘脑室周围后核中检测到，免疫反应阳性纤维分布在各个脑区和垂体中。我们还检测gfLPXRFa-免疫反应性的垂体竞争性酶联免疫吸附试验结合反相HPLC。这三种LPXR-Famide肽刺激FSH、LH和GH的释放，但不影响催乳素（PRL）和促甲状腺素（SL）从培养的垂体细胞的释放。这些结果表明，新的鱼下丘脑LPXR-Famide肽存在于红鲑鱼的脑和垂体中，并刺激促性腺激素和GH从垂体的释放.
We recently identified a cDNA encoding three novel fish hypothalamic neuropeptides, having LPXRF-NH2 from the goldfish brain. In this Study, to clarify the physiological functions of these three LPXRFamide peptides (gfLPXRFa-1, -2, and -3), we analysed the localisation and hypophysiotrophic activity of these peptides using sockeye salmon, Oncorhynchus nerka, in which immunoassay systems for several interior pituitary hormones have been developed. gfLPXKFa-immunoreactive cell bodies were detected in the nucleus posterioris periventricularis of the hypothalamus and immunooreactive fibres were distributed in various brain regions and the pituitary. We also detected gfLPXRFa-immunoreactivity in the pituitary by competitive enzyme-linked immunosorbent assay combined with reversed-phase HPLC. These three gfLPXRFamide peptides stimulated the release of FSH, LH and GH, but did not affect the release of prolactin (PRL) and soiatolactin (SL) from cultured pituitary cells ' These results suggest that novel fish hypothalamic LPXR- Famide peptides exist in the brain and pituitary of sockeye salmon and stimulate the release of gonadotrophins and GH from the pituitary.