Long-term evaluation of vascular grafts with circumferentially aligned microfibers in a rat abdominal aorta replacement model
Long-term evaluation of vascular grafts with circumferentially aligned microfibers in a rat abdominal aorta replacement model
复制标题
大鼠腹主动脉置换模型中周向排列微纤维血管移植物的长期评估
DOI：
10.1002/jbm.b.34076
复制
发表时间：
2018-10-01
影响因子：
3.4
通讯作者：
Kong, Deling
中科院分区：
文献类型：
--
作者：
Li, Wen;Chen, Jingrui;Kong, Deling
植入物在小动物模型中的长期结果可用于优化移植物的设计，以进一步促进组织再生。在以前的研究中，我们制作了一个聚（-己内酯）（PCL）双层血管移植物由一个内部层与圆周排列的微纤维和一个外部层与随机纳米纤维。移植物植入大鼠腹主动脉3个月后，血管平滑肌细胞（VSMCs）成功再生。在此，我们研究了双层移植物在相同模型中的长期（18个月）性能。所有移植物均通畅。未发生血栓形成、动脉瘤或狭窄。内皮完整。但大多数血管平滑肌细胞沿周向迁移至移植物管腔表面，形成厚度均匀的新生内膜。因此，细胞外基质包括胶原蛋白、弹性蛋白和糖胺聚糖在新生内膜层中显示高密度，而在移植物壁中由于PCL的不完全降解而显示低密度。移植物中出现少量钙化。新生动脉的收缩和舒张功能几乎消失。这些数据表明，在结构设计的基础上，还应考虑移植物的其他因素，以实现长期植入后类似于自体血管的再生新动脉。(C)2018 Wiley Periodicals，Inc.
Long-term results of implants in small animal models can be used to optimize the design of grafts to further promote tissue regeneration. In previous study, we fabricated a poly(-caprolactone) (PCL) bi-layered vascular graft consisting of an internal layer with circumferentially aligned microfibers and an external layer with random nanofibers. The circumferentially oriented vascular smooth muscle cells (VSMCs) were successfully regenerated after the grafts were implanted in rat abdominal aorta for 3 months. Here we investigated the long-term (18 months) performance of the bi-layered grafts in the same model. All the grafts were patent. No thrombosis, aneurysm, or stenosis occurred. The endothelium maintained complete. However, most of circumferentially oriented VSMCs migrated to luminal surface of the grafts to form a neointima with uniform thickness. Accordingly, extracellular matrix including collagen, elastin, and glycosaminoglycan displayed high density in neointima layer while with low density in the grafts wall because of the incomplete degradation of PCL. A small amounts of calcification occurred in the grafts. The contraction and relaxation function of regenerated neoartery almost disappeared. These data indicated that based on the structure design, many other factors of grafts should be considered to achieve the regenerated neoartery similar to the native vessels after long-term implantation. (C) 2018 Wiley Periodicals, Inc.