Mechanical Loading Improves Tendon Bone Healing in a Rabbit Anterior Cruciate Ligament Reconstruction Model by Promoting Proliferation and Matrix Formation of Mesenchymal Stem Cells and Tendon Cells
Mechanical Loading Improves Tendon Bone Healing in a Rabbit Anterior Cruciate Ligament Reconstruction Model by Promoting Proliferation and Matrix Formation of Mesenchymal Stem Cells and Tendon Cells
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机械负荷通过促进间充质干细胞和肌腱细胞的增殖和基质形成来改善兔前十字韧带重建模型的肌腱骨愈合
DOI：
10.1159/000460005
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发表时间：
2017-01-01
影响因子：
--
通讯作者：
Kang, Yifan
中科院分区：
文献类型：
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作者：
Song, Fanglong;Jiang, Dawei;Kang, Yifan
背景/目标：本研究探讨了机械应力对兔前交叉韧带（ACL）重建模型中腱-骨愈合的影响，以及对骨髓间充质干细胞（BMSCs）和肌腱细胞（TC）共培养中细胞增殖和基质形成的影响。研究方法：通过组织学分析、生物力学测试和腱-骨界面基因表达评价持续被动运动（CPM）治疗对兔ACL重建模型腱-骨愈合的影响。此外，还研究了机械拉伸对BMSC/TC共培养中细胞增殖和基质合成的影响。结果如下：术后CPM治疗显著增强了腱-骨愈合，如纤维软骨量增加、极限负荷升高至失败水平以及腱-骨连接处I型胶原、碱性磷酸酶、骨桥蛋白、腱生蛋白C和腱调节蛋白的基因表达上调所证明的。另外，机械牵张处理后的BMSC/TC共培养细胞的增殖率和I型胶原、III型胶原、碱性磷酸酶、骨桥蛋白、腱生蛋白C和腱调节蛋白的表达均高于对照组。结论：这些结果表明，机械刺激可以增强局部前体细胞的增殖和分化，从而增强BMSCs和TC在肌腱-骨愈合中的再生潜力。
Background/Aims: This study investigated the effect of mechanical stress on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model as well as cell proliferation and matrix formation in co-culture of bone-marrow mesenchymal stem cells (BMSCs) and tendon cells (TCs). Methods: The effect of continuous passive motion (CPM) therapy on tendon-bone healing in a rabbit ACL reconstruction model was evaluated by histological analysis, biomechanical testing and gene expressions at the tendon-bone interface. Furthermore, the effect of mechanical stretch on cell proliferation and matrix synthesis in BMSC/TC co-culture was also examined. Results: Postoperative CPM therapy significantly enhanced tendon-bone healing, as evidenced by increased amount of fibrocartilage, elevated ultimate load to failure levels, and up-regulated gene expressions of Collagen I, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin at the tendon-bone junction. In addition, BMSC/TC co-culture treated with mechanical stretch showed a higher rate of cell proliferation and enhanced expressions of Collagen I, Collagen III, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin than that of controls. Conclusion: These results demonstrated that proliferation and differentiation of local precursor cells could be enhanced by mechanical stimulation, which results in enhanced regenerative potential of BMSCs and TCs in tendon-bone healing.