Shape selectivity for constrained solutes in reversed-phase liquid chromatography
Shape selectivity for constrained solutes in reversed-phase liquid chromatography
复制标题
DOI：
10.1021/ac9908187
复制
发表时间：
1999-11-01
影响因子：
7.4
通讯作者：
Wise, SA
中科院分区：
文献类型：
--
作者：
Sander, LC;Pursch, M;Wise, SA
在反相液相色谱（RPLC）中，分离极性相似的化合物混合物对分析人员来说是一个重大的挑战。此类化合物的示例包括存在于环境样品中的几何异构体（例如，多环芳烃、多环芳烃硫杂环和多氯联苯同系物）和具有生物学意义的化合物（例如，类胡萝卜素和类固醇）。一般来说，具有刚性、明确的分子形状的化合物最好使用具有增强形状选择性特征的色谱柱分离。这个观点提出了一个概述柱的性质，影响形状选择性的约束溶质。描述了表征固定相结构的方法，并将研究结果与色谱性能相关联。最后，提出了与观察到的保留行为相一致的形状识别保留模型。对RPLC中形状识别效果的认识将有助于某些难解化合物的方法开发。
In reversed-phase Liquid chromatography (RPLC), the separation of compound mixtures of similar polarity can present a significant challenge for the analyst. Examples of such compounds include geometric isomers present kin environmental samples (e.g., polycyclic aromatic hydrocarbons, polycyclic aromatic sulfur heterocycles, and polychlorinated biphenyl congeners) and compounds of biological significance (e.g., carotenoids and steroids). In general, compounds with rigid, well-defined molecular shape are best separated using a column with enhanced shape selectivity characteristics. This perspective presents an overview of column properties that influence shape selectivity for constrained solutes. Approaches to the characterization of stationary-phase structure are described, and the findings are correlated with chromatographic performance. Finally, retention models of shape discrimination are presented that are consistent with observed retention behavior. An appreciation for shape recognition effects in RPLC will facilitate method development for certain classes of difficult to resolve compounds.