Effective anaerobic treatment of fresh leachate from MSW incineration plant and dynamic characteristics of microbial community in granular sludge
Effective anaerobic treatment of fresh leachate from MSW incineration plant and dynamic characteristics of microbial community in granular sludge
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垃圾焚烧厂新鲜渗滤液的有效厌氧处理及颗粒污泥微生物群落动态特征
DOI：
10.1007/s00253-013-4792-2
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发表时间：
2013-03
影响因子：
5
通讯作者：
Sun Dezhi
中科院分区：
文献类型：
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作者：
Ye Jiexu;Mu Yongjie;Qiu Bin;Sun Dezhi
采用实验室规模的膨胀颗粒污泥床（EGSB）反应器处理城市生活垃圾焚烧厂新鲜渗滤液。反应器在中温温度（33 °C）下操作118天。渗滤液进水化学需氧量（COD）逐渐上升至70,000 mg/L以上，有机负荷率上升至18 kg COD/（m3·d）。当以渗滤液为原料时，反应器的COD平均去除率达到86.7%，表明EGSB工艺处理渗滤液的可行性。采用变性梯度凝胶电泳、克隆文库和实时定量聚合酶链反应等方法，构建了试运行期间反应器污泥中的微生物群落。古菌优势类群为甲烷八叠球菌，占62.3%。在整个操作过程中，真细菌的优势类群为厚壁菌，其比例从> 50%增加到81.2%。几乎所有的厚壁菌属的操作分类单位属于梭菌目，具有特征性的孢子形成。以原水渗滤液为进料时，反应器内微生物种群多样性较低。结合EGSB反应器的运行状况，探讨了厌氧颗粒污泥中微生物群落的动态变化规律。
We investigated the treatment of fresh leachate from municipal solid waste incineration plants with high-strength organics using a lab-scale expanded granular sludge bed (EGSB) reactor. The reactor was operated at a mesophilic temperature (33 °C) for 118 days. The influent chemical oxygen demand (COD) of the leachate gradually increased to over 70,000 mg/L, and the organic loading rate increased to 18 kg COD/(m3day). An average COD removal efficiency of 86.7 % was achieved when the reactor was fed with raw leachate, which suggests the feasibility of the EGSB process for leachate treatment. The microbial communities in the sludge from the reactor during the trial operation were constructed by denaturing gradient gel electrophoresis, clone libraries, and real-time quantitative polymerase chain reaction. The dominant group for archaea wasMethanosaeta, with 68.4 % proportion at the start of the operation, and then changed toMethanosarcina, with a proportion of 62.3 %, after 118 days of operation. The dominant group of eubacteria was confirmed to beFirmicutesthroughout the operation process, with the proportion increasing from >50 to 81.2 %. Almost all the operational taxonomic units ofFirmicutesbelonged to the orderClostridiales, with characteristic spore formation. The microbial diversity of the population was low under raw leachate as feed in the reactor. The dynamics of the microbial community in the anaerobic granular sludge was discussed relating with the operating status of the EGSB reactor.
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影响因子：
4.4
作者：
A. D. Silva;M. Varesche;E. Foresti;M. Zaiat
通讯作者：
A. D. Silva;M. Varesche;E. Foresti;M. Zaiat
影响因子：
12.8
作者：
H. Yu;J. Tay;H. H. Fang-H.
通讯作者：
H. Yu;J. Tay;H. H. Fang-H.
影响因子：
11.4
作者：
R. Kettunen;Taina H. Hoilijoki;J. Rintala
通讯作者：
R. Kettunen;Taina H. Hoilijoki;J. Rintala
影响因子：
11.4
作者：
Jianyong Liu;Jun Hu;Jiangping Zhong;Jinghuan Luo;A. Zhao;Feng Liu;R. Hong;G. Qian;Z. Xu
通讯作者：
Jianyong Liu;Jun Hu;Jiangping Zhong;Jinghuan Luo;A. Zhao;Feng Liu;R. Hong;G. Qian;Z. Xu
影响因子：
--
作者：
SHANNON, CE
通讯作者：
SHANNON, CE