Highly sensitive and selective detection of silver(I) in aqueous solution with silver(I)-specific DNA and Sybr Green I.
Highly sensitive and selective detection of silver(I) in aqueous solution with silver(I)-specific DNA and Sybr Green I.
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DOI：
10.1039/c3an36496h
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发表时间：
2013-03
期刊：
影响因子：
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通讯作者：
Qing Yang;Fan Li;Y. Huang;Hui Xu;Linsheng Tang;Lihua Wang;C. Fan
中科院分区：
文献类型：
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作者：
Qing Yang;Fan Li;Y. Huang;Hui Xu;Linsheng Tang;Lihua Wang;C. Fan
基于银离子诱导富含胞嘧啶的单链DNA构象变化，提出了一种灵敏、选择性检测银离子Ag（+）的无标记荧光开启方法。银特异性寡核苷酸（SSO）经历从单链DNA（ssDNA）到C-Ag（+）-C介导的发夹结构的结构转变，其可被双链DNA（dsDNA）嵌入剂Sybr绿色I（SG）识别。Ag（+）检测的线性响应范围为1 nM至100 nM。这里提出的方法是高度灵敏的，并获得了1 nM的检测限。更重要的是，这种光学方法被成功地用于识别复杂的样品混合物。
We report a label-free fluorescence turn-on approach for the sensitive and selective sensing of silver ion Ag(+) based on Ag(+) induced conformational change of cytosine-rich single-stranded DNA. A silver specific oligonucleotide (SSO) undergoes a structural transition from single-stranded DNA (ssDNA) to a C-Ag(+)-C mediated hairpin structure, which can be recognized by a double-stranded DNA (dsDNA) intercalator Sybr Green I (SG). The linear response range for Ag(+) detection was from 1 nM to 100 nM. The method presented here is highly sensitive and a limit of detection of 1 nM was obtained. More importantly, this optical method was successfully used to identify complex sample mixtures.