Immunolocalization of an inwardly rectifying K+ channel, K-AB-2 (Kir4.1), in the basolateral membrane of renal distal tubular epithelia
Immunolocalization of an inwardly rectifying K+ channel, K-AB-2 (Kir4.1), in the basolateral membrane of renal distal tubular epithelia
复制标题
DOI：
10.1016/0014-5793(96)00502-9
复制
发表时间：
1996-06-10
期刊：
影响因子：
3.5
通讯作者：
Kurachi, Y
中科院分区：
文献类型：
--
作者：
Ito, M;Inanobe, A;Kurachi, Y
本实验观察了K-AB-2（Kir4.1）在大鼠肾脏的免疫定位。K-AB-2是一种具有ATP结合结构域的内向整流钾离子通道。K-AB-2是第一个定位于肾小管上皮细胞基底侧膜的K+通道。这一发现提示K-AB-2可能参与了向Na+-K+泵提供K+的过程，而Na+-K+泵在肾小管上皮细胞基底侧膜上是丰富的。以及维持这些细胞的深负膜电位。
Immunolocalization of K-AB-2 (Kir4.1), an inwardly rectifying K+ channel with a putative ATP-binding domain, was examined in rat kidney where expression of K-AB-2 mRNA was previously shown, Anti-K-AB-2 antibody was raised in rabbit and then affinity-purified. An immunohistochemical study revealed that K-AB-2 immunoreactivity was detected specifically in the basolateral membrane of distal tubular epithelia, Therefore, K-AB-2 is the first K+ channel shown to be localized in the basolateral membrane of renal epithelia, The finding suggests that K-AB-2 may contribute to supplying K+ to the Na+-K+ pump, which is abundant in the basolateral membrane of distal tubular epithelia, as well as to maintenance of the deep negative membrane potential of these cells.