Smoothelin contains a novel actin cytoskeleton localization sequence with similarity to troponin T
Smoothelin contains a novel actin cytoskeleton localization sequence with similarity to troponin T
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DOI：
10.1002/jcb.10143
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Leonhardt, H
中科院分区：
文献类型：
--
作者：
Quensel, C;Krämer, J;Leonhardt, H
Smoothelin是一种细胞骨架蛋白，存在于血管平滑肌细胞中的大亚型和转录起始点下游的内脏小亚型中。利用与绿色荧光蛋白的融合，我们可以证明这两种平滑蛋白亚型都定位于含有肌动蛋白的细丝，并定位了两个结构域，每个结构域都足以定位于肌动蛋白细胞骨架。第一个结构域位于光滑蛋白的血管特异的N-末端一半，第二个结构域位于共同的C-末端一半。第二个结构域与肌钙蛋白T参与肌动蛋白细丝结合的结构域有明显的序列相似性。这些结果表明，平滑蛋白亚型的组织特异性表达可能与平滑肌细胞不同的收缩特性有关。J.细胞。生物化学。85：403-409,2002。(C)2002年Wiley-Liss，Inc.
Smoothelin, a cytoskeletal protein, exists in a large isoform specifically expressed in vascular smooth muscle cells, and a small visceral isoform generated by a downstrearn transcriptional start site. Using fusions to the green fluorescent protein, we could show that both smoothelin isoforms are localized at actin containing filaments and mapped two domains that are each sufficient for localization at the actin cytoskeleton. The first domain is located in the vascular-specific, N-terminal half of smoothelin and the second in the common, C-terminal half. The second domain shares clear sequence similarity with a domain of troponin T involved in actin filament association. These results suggest that the tissue-specific expression of smoothelin isoforms might contribute to the different contractile properties of smooth muscle cells. J. Cell. Biochem. 85: 403-409, 2002. (C) 2002 Wiley-Liss, Inc.