EGRI and FOSB gene expressions in cancer stroma are independent prognostic indicators for epithelial ovarian cancer receiving standard therapy
EGRI and FOSB gene expressions in cancer stroma are independent prognostic indicators for epithelial ovarian cancer receiving standard therapy
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DOI：
10.1002/gcc.21916
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发表时间：
2012-03-01
影响因子：
3.7
通讯作者：
Aoki, Daisuke
中科院分区：
文献类型：
--
作者：
Kataoka, Fumio;Tsuda, Hiroshi;Aoki, Daisuke
基质成分与癌细胞相互作用以促进生长和转移。本研究的目的是鉴定间质中表达的基因，这些基因可以提供上皮性卵巢癌（EOC）的预后信息。共纳入74例患者。我们进行了基因表达谱分析，并使用RT-PCR和免疫组织化学确认阵列数据。通过基因芯片分析，筛选出52个与无进展生存期（PFS）相关的候选基因（P < 0.005）。进一步分析早期生长反应1（EGFR 1）和FBJ鼠骨肉瘤病毒癌基因同源物B（FOSB）基因的表达。RT-PCR和多因素分析证实EGR 1和FOSB在卵巢癌间质中的表达以及EGR 1在卵巢癌中的表达是影响卵巢癌预后的独立因素。免疫组化显示，EGFR 1蛋白定位于癌细胞和α-平滑肌肌动蛋白阳性基质成纤维细胞中。间质细胞中EGR 1和FOSB的表达以及癌细胞中EGR 1的表达是EOC的预后指标。(c)2011 Wiley Periodicals，Inc.
Stromal components interact with cancer cells to promote growth and metastasis. The purpose of this study was to identify genes expressed in stroma, which could provide prognostic information in epithelial ovarian cancer (EOC). Seventy-four patients were included. We performed gene expression profiling and confirmed array data using RT-PCR and immunohistochemistry. By microarray analysis, 52 candidate genes associated with progression free survival (PFS) were identified (P < 0.005). Expression of the early growth response 1 (EGR1) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) genes was further analyzed. Array data were confirmed by RT-PCR and multivariate analysis demonstrated that both EGR1 and FOSB expression in cancer stroma, and EGR1 expression in cancer are independent prognostic factors in EOC. Immunohistochemically, EGR1 protein is localized in cancer cells and a-smooth muscle actin positive stromal fibroblasts. The EGR1 and FOSB expression in stromal cells and EGR1 expression in cancer cells are prognostic indicators in EOC. (c) 2011 Wiley Periodicals, Inc.