Identification of cells migrating from the thecal layer of ovarian follicles
Identification of cells migrating from the thecal layer of ovarian follicles
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DOI：
10.1007/s00441-013-1621-y
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发表时间：
2013-07-01
影响因子：
3.6
通讯作者：
Spears, Norah
中科院分区：
文献类型：
--
作者：
Campbell, Lisa;Trendell, Jenny;Spears, Norah
在哺乳动物卵巢中，卵母细胞包含在卵泡内。这些细胞存在于被支持细胞包围的卵母细胞中：内层颗粒细胞和外层卵泡膜细胞被基膜分开。在任何时候，都存在一个正在发育的卵泡群，其中只有一小部分特定的卵泡被选择继续发育到排卵，其余的则因卵泡闭锁而死亡。在这里，我们使用体外方法来研究培养中两个卵泡之间的相互作用（卵泡共培养）。我们发现，当两个单独的卵泡在培养中一起生长时，细胞和细胞过程从一个卵泡的外膜层迁移到另一个共同培养的卵泡的膜层。这些细胞被鉴定为主要含有内皮细胞但也含有神经元细胞的混合群体。两者都能够穿过卵巢间质迁移，与其他卵泡的基底层以及来自这些其他卵泡的类似细胞接触。这些细胞的网络可能参与卵泡间的通讯和排卵卵泡选择的协调。
In the mammalian ovary, oocytes are contained within ovarian follicles. These consist in an oocyte surrounded by supporting cells: an inner layer of granulosa cells and an outer layer of thecal cells separated by a basal lamina. At any one time, a developing cohort of follicles exists, from which only a small species-specific number are selected for continued development towards ovulation, with the remainder dying by follicular atresia. Here, we use in vitro methods to study interactions between two follicles in culture (follicle co-cultures). We show that, when two individual follicles are grown together in culture, cells and cellular processes migrate from the outer thecal layer of one follicle to the thecal layer of the other co-cultured follicle. These cells are identified as a mixed population containing primarily endothelial but also neuronal cells. Both are able to migrate through the ovarian interstitum, making contact with the basal lamina of other follicles and with similar cells from these other follicles. Networks of such cells might be involved in interfollicular communication and in the coordination of follicle selection for ovulation.