Ultrasensitive indirect competitive ELISA and strip sensor for detection of furazolidone metabolite in animal tissues
Ultrasensitive indirect competitive ELISA and strip sensor for detection of furazolidone metabolite in animal tissues
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用于检测动物组织中呋喃唑酮代谢物的超灵敏间接竞争 ELISA 和条形传感器
DOI：
10.1080/09540105.2017.1337083
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Yuan, Jian
中科院分区：
文献类型：
--
作者：
Xing, Changrui;Jing, Xuexue;Yuan, Jian
硝基呋喃类药物是水产养殖中非法使用的一类抗生素或抗菌剂。本文建立了检测呋喃唑酮代谢物3-氨基-2-恶唑烷酮（AOZ）的间接竞争酶联免疫吸附试验和试纸条传感器。这两种方法均具有较高的敏感性和特异性。半抑制浓度为0.074 ng/mL的免疫吸附法检测猪肉、鸡肉、鱼、虾、猪肝和鸡肝中AOZ的回收率在79.9%~ 119.8%之间。 该方法的检测限为0.05 ng/g，临界值为0.3 ng/g。 与其他硝基呋喃类化合物、代谢物及其他化合物的交叉反应性均小于0.1%。样品回收率为97.3%~ 115.5%。这两种方法均可用于动物组织中痕量呋喃唑酮代谢物的现场检测。
ABSTRACT Nitrofurans are a class of drugs, which are typically used as antibiotics or antimicrobials illegally in aquiculture. In this paper, indirect competitive enzyme-linked immunosorbent assay and strip sensor were developed for detection of furazolidone metabolite, 3-amino-2-oxazolidinone (AOZ). These two methods could detect AOZ with high sensitivity and specificity. The immunosorbent assay with the half-inhibitory concentration of 0.074 ng/mL has a good recovery from 79.9% to 119.8% for detection of AOZ in pork, chicken, fish, shrimp, pork liver and chicken liver. Based on the developed strip sensor, the detection limit was 0.05 ng/g by the naked eyes and the cut-off value was 0.3 ng/g. The cross-reactivity with other nitrofurans, metabolites and some other compounds was all less than 0.1%. The sample recoveries ranged from 97.3% to 115.5%. Both these methods could be used for on-site detection of furazolidone metabolite at trace level in animal tissues.