Plastid inheritance and plastome-genome incompatibility of intergeneric hybrids between Menziesia and Rhododendron
Plastid inheritance and plastome-genome incompatibility of intergeneric hybrids between Menziesia and Rhododendron
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DOI：
10.2503/jjshs.74.318
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Handa, T
中科院分区：
文献类型：
--
作者：
Kita, K;Kurashige, Y;Handa, T
门齐虫和杜鹃花的属间杂交产生许多白化或灰绿色的幼苗。为了阐明导致白化表型的因素，我们采用PCR - RFLP分析方法，分别对门齐虫属内两个属间杂交种和一个种内杂交种进行遗传分析。白化和淡绿色属间子代均含有杜鹃花的ptDNA，而绿色子代含有门齐孢子虫的ptDNA，说明杜鹃花质体与门齐孢子虫核基因组不相容。此外，当多花分枝杆菌作为母本使用时，大多数后代将其质体传递给父本。门齐虫-杜鹃花杂交的质粒-基因组不亲和性似乎与ptDNA遗传无关。
Intergeneric hybridization between Menziesia and Rhododendron generates many albino or pate - green seedlings. To clarify the factors that cause albino phenotype, we analyzed the inheritance of plastid (pt) DNA by PCR - RFLP analysis on the intergeneric hybrids between two genera and an intraspecific hybrid within Menziesia by reciprocal cross-pollination, respectively. All albino and pale-green intergeneric progenies contained the ptDNA of Rhododendron, whereas green progenies contained that of Menziesia, indicating that Rhododendron plastome is incompatible with Menziesia nuclear genome. Moreover, when M. multiflora was used as a female parent, most progenies transmitted their plastids paternally. Plastome-genome incompatibility seems to be independent from ptDNA inheritance in Menziesia - Rhododendron hybrids.