Gene expression profiles of Spo11-/- mouse testes with spermatocytes arrested in meiotic prophase I.
Gene expression profiles of Spo11-/- mouse testes with spermatocytes arrested in meiotic prophase I.
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DOI：
10.1530/rep.1.00997
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发表时间：
2006-07
期刊：
影响因子：
3.8
通讯作者：
N. Smirnova;P. Romanienko;P. Khil;R. Camerini‐Otero
中科院分区：
文献类型：
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作者：
N. Smirnova;P. Romanienko;P. Khil;R. Camerini‐Otero
Spo 11是一种减数分裂特异性蛋白，在多种生物中引入染色体DNA双链断裂并启动减数分裂重组。小鼠空Spo 11精母细胞未能突触染色体和进展超出偶线期减数分裂。我们分析了Spo 11（-/ -）成年和幼年野生型睾丸的基因表达谱，以描述Spo 11敲除导致减数分裂阻滞前后表达的基因。这些基因的特点是使用基因本体数据库。为了关注减数分裂相关基因，我们对出生15天的小鼠Spo 11（-/ -）和野生型睾丸进行了比较基因表达分析，此时精母细胞刚刚进入粗线期。我们发现，Spo 11的敲除导致参与减数分裂重组（Hop 2，Brca 2，Mnd 1，FancG）和减数分裂检查点（细胞周期蛋白B2，Cks 2）的基因的表达水平发生显著变化，但不影响编码联会复合体蛋白组分的基因。最后，我们发现了受Spo 11基因破坏影响的未知基因，因此可能专门参与减数分裂和精子发生。
Spo11, a meiosis-specific protein, introduces double-strand breaks on chromosomal DNA and initiates meiotic recombination in a wide variety of organisms. Mouse null Spo11 spermatocytes fail to synapse chromosomes and progress beyond the zygotene stage of meiosis. We analyzed gene expression profiles in Spo11(-/ -)adult and juvenile wild-type testis to describe genes expressed before and after the meiotic arrest resulting from the knocking out of Spo11. These genes were characterized using the Gene Ontology data base. To focus on genes involved in meiosis, we performed comparative gene expression analysis of Spo11(-/ -)and wild-type testes from 15-day mice, when spermatocytes have just entered pachytene. We found that the knockout of Spo11 causes dramatic changes in the level of expression of genes that participate in meiotic recombination (Hop2, Brca2, Mnd1, FancG) and in the meiotic checkpoint (cyclin B2, Cks2), but does not affect genes encoding protein components of the synaptonemal complex. Finally, we discovered unknown genes that are affected by the disruption of the Spo11 gene and therefore may be specifically involved in meiosis and spermatogenesis.