Magnesium promotes bone formation and angiogenesis by enhancing MC3T3-E1 secretion of PDGF-BB
Magnesium promotes bone formation and angiogenesis by enhancing MC3T3-E1 secretion of PDGF-BB
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镁通过增强MC3T3-E1分泌PDGF-BB来促进骨形成和血管生成
DOI：
10.1016/j.bbrc.2020.05.113
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发表时间：
2020-08-06
影响因子：
3.1
通讯作者：
Guo, Zheng
中科院分区：
文献类型：
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作者：
Liu, Wenwen;Guo, Shuo;Guo, Zheng
虽然镁及其合金是骨科植入物的候选者，但它们可以有效地促进骨生成和血管生成。但由于镁的可降解性，不同浓度的镁离子对细胞的影响不同，影响了镁植入物的安全性。镁促进骨生成和血管生成的细胞和分子机制尚不清楚，这进一步影响了其临床应用。本研究用不同浓度的镁离子处理HUVECs，发现浓度在1 ~ 5 mM之间，尤其是5 mM，最适合HUVECs的培养。通过基因测序、RT-PCR、ELISA和Western blot分析，发现镁可促进MC 3 T3-E1的表达和PDGF-BB的分泌。然后，成骨相关试验和血管生成相关试验发现，镁促进MC 3 T3-E1分泌PDGF-BB，不仅提高成骨细胞的成骨分化能力，而且有效促进HUVEC的血管生成能力。(C)2020由Elsevier Inc.出版。
Although magnesium and its alloys are candidates for orthopedic implants, they can effectively promote osteogenesis and angiogenesis. However, due to the degradability of magnesium, different concentrations of magnesium ions have different effects on cells, which affects the safety of magnesium implants. The cellular and molecular mechanisms by which magnesium promotes osteogenesis and angiogenesis are still unclear, which further affects its clinical use. In this study, HUVECs were treated with different concentrations of magnesium ions, and the concentration between 1 and 5 mM, especially 5 mM, was most suitable for cultivation of HUVECs. Using gene sequencing, RT-PCR, ELISA and Western blot analysis, we found that magnesium can promote MC3T3-E1 expression and secretion of PDGF-BB. Then, osteogenesis-related tests and angiogenesis-related tests found that magnesium promotes the secretion of PDGF-BB by MC3T3-E1 not only to improve the osteogenic differentiation ability of osteoblasts but also to effectively promote the angiogenic ability of HUVECs. (C) 2020 Published by Elsevier Inc.