POLYMER-STIMULATED LIGATION - ENHANCED BLUNT-END OR COHESIVE-END LIGATION OF DNA OR DEOXYRIBOOLIGONUCLEOTIDES BY T4-DNA LIGASE IN POLYMER-SOLUTIONS
POLYMER-STIMULATED LIGATION - ENHANCED BLUNT-END OR COHESIVE-END LIGATION OF DNA OR DEOXYRIBOOLIGONUCLEOTIDES BY T4-DNA LIGASE IN POLYMER-SOLUTIONS
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DOI：
10.1093/nar/11.22.7853
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
ZIMMERMAN, SB
中科院分区：
文献类型：
--
作者：
PHEIFFER, BH;ZIMMERMAN, SB
在存在高浓度的各种非特异性聚合物如聚乙二醇、Ficoll、牛血浆白蛋白或糖原的情况下，T4 DNA连接酶对DNA的平端和粘性端连接的速率增加了几个数量级。小的自身互补的寡脱氧核糖核苷酸的平端连接也被刺激。聚乙二醇6000在这样的系统中的使用的特点是在一些细节。我们提出了一些条件，这些条件要么能大大增加线性连接产物的形成速率和大小，要么能使环状连接产物的数量有较小但显著的改善。
The rates of blunt-end and cohesive-end ligation of DNA by T4 DNA ligase are Increased by orders of magnitude in the presence of high concentrations of a variety of nonspecific polymers such as polyethylene glycol, Ficoll, bovine plasma albumin, or glycogen. Blunt-end ligation of small self-complementary oligodeoxyribonucleotides is also stimulated. The use of polyethylene glycol 6000 In such systems is characterized In some detail. Conditions are suggested which either greatly increase the rate of formation of and size oflinearligation products or which allow smallerbut significant Improvements in the amounts ofcircular ligation products.