A new splice variant of the mouse SIRT3 gene encodes the mitochondrial precursor protein.
A new splice variant of the mouse SIRT3 gene encodes the mitochondrial precursor protein.
复制标题
小鼠SIRT3基因的新剪接变体编码线粒体前体蛋白。
DOI：
10.1371/journal.pone.0004986
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Spelbrink JN
中科院分区：
文献类型：
--
作者：
Cooper HM;Huang JY;Verdin E;Spelbrink JN
Mammals have seven NAD-dependent protein deacetylases. These proteins, called sirtuins, are homologous to yeast Sir2, and are emerging as important regulators of lifespan and intermediary metabolism. Three mammalian sirtuins, SIRT3-5 are mitochondrial. Sirtuins are highly conserved between species, yet mouse SIRT3 was reported to be markedly shorter than its human counterpart and to lack the N-terminal mitochondrial targeting signal present in the human protein. We have isolated a novel mouse SIRT3 splice variant. This cDNA contains two translation initiation codons upstream of the originally reported start site. We show, using immunofluorescence and protein expression analysis that these longer variants are expressed and efficiently targeted to mitochondria, and that the processed forms of these longer variants are identical in size to the endogenous mouse SIRT3. We also show that the previously described form of SIRT3 is not mitochondrial. Our observations point to a high level of conservation of SIRT3 as a mitochondrial protein in mice and human and indicate that several previous studies, which addressed mouse Sirt3 function, need to be re-evaluated.
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影响因子：
10.5
作者：
Scher, Michael B.;Vaquero, Alejandro;Reinberg, Danny
通讯作者：
Reinberg, Danny
DOI：
10.1083/jcb.200205057
发表时间：
2002-08-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Schwer B;North BJ;Frye RA;Ott M;Verdin E
通讯作者：
Verdin E
影响因子：
5.6
作者：
Schlicker, Christine;Gertz, Melanie;Steegborn, Clemens
通讯作者：
Steegborn, Clemens
影响因子：
10.5
作者：
Kaeberlein, M;McVey, M;Guarente, L
通讯作者：
Guarente, L
影响因子：
4.8
作者：
Spelbrink, JN;Toivonen, JM;Jacobs, HT
通讯作者：
Jacobs, HT