Generic and specific transcriptional responses to different weak organic acids in anaerobic chemostat cultures of Saccharomyces cerevisiae
Generic and specific transcriptional responses to different weak organic acids in anaerobic chemostat cultures of Saccharomyces cerevisiae
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DOI：
10.1111/j.1567-1364.2007.00242.x
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发表时间：
2007-09-01
影响因子：
3.2
通讯作者：
van Maris, Antonius J. A.
中科院分区：
文献类型：
--
作者：
Abbott, Derek A.;Knijnenburg, Theo A.;van Maris, Antonius J. A.
研究了4种弱有机酸(苯甲酸、山梨酸、醋酸盐和丙酸)在厌氧、葡萄糖限制的恒化培养中对酿酒酵母的转录反应。为了能够定量比较酸的反应，选择了酸的浓度，使葡萄糖的生物量减少50%。达到这一产率所需的每种酸的浓度与膜亲和力呈负相关。微阵列分析显示，每种酸导致数百个转录本与没有添加有机酸的参考培养物相比变化两倍以上。然而，只有14个基因在对所有酸的反应中持续上调。中等亲油性的化合物苯甲酸盐和山梨酸酯，以及程度较小的脂亲性酸醋酸酯和丙酸酯表现出重叠的转录反应。对过度表达的功能类别和上游调控元件的统计分析表明，对强亲脂酸的反应集中在与细胞壁相关的基因上，而醋酸和丙酸对膜相关的运输过程有更强的影响。酿酒酵母对弱有机酸表现出最小的通用转录反应和广泛的特异性反应，这一事实对于解释和控制食品和工业发酵过程中的弱酸毒性具有重要意义。
Transcriptional responses to four weak organic acids (benzoate, sorbate, acetate and propionate) were investigated in anaerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae. To enable quantitative comparison of the responses to the acids, their concentrations were chosen such that they caused a 50% decrease of the biomass yield on glucose. The concentration of each acid required to achieve this yield was negatively correlated with membrane affinity. Microarray analysis revealed that each acid caused hundreds of transcripts to change by over twofold relative to reference cultures without added organic acids. However, only 14 genes were consistently upregulated in response to all acids. The moderately lipophilic compounds benzoate and sorbate and, to a lesser extent, the less lipophilic acids acetate and propionate showed overlapping transcriptional responses. Statistical analysis for overrepresented functional categories and upstream regulatory elements indicated that responses to the strongly lipophilic acids were focused on genes related to the cell wall, while acetate and propionate had a stronger impact on membrane-associated transport processes. The fact that S. cerevisiae exhibits a minimal generic transcriptional response to weak organic acids along with extensive specific responses is relevant for interpreting and controlling weak acid toxicity in food products and in industrial fermentation processes.