Development of Synechocystis sp. PCC 6803 as a phototrophic cell factory.
Development of Synechocystis sp. PCC 6803 as a phototrophic cell factory.
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DOI：
10.3390/md11082894
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发表时间：
2013-08-13
期刊：
影响因子：
5.4
通讯作者：
Zhang F
中科院分区：
文献类型：
--
作者：
Yu Y;You L;Liu D;Hollinshead W;Tang YJ;Zhang F
蓝藻在生态学和地球化学中具有重要的作用。一种模式蓝细菌物种是单细胞蓝细菌集胞藻属PCC 6803。这个物种非常适合基因改造。它的基因组已被测序，许多系统生物学和分子生物学工具可用于研究这种细菌。最近，研究人员投入了大量精力来了解和改造这种细菌，以利用阳光和二氧化碳生产化学品和生物燃料。为了展示我们的观点，这种蓝藻作为一个光合作用为基础的底盘的应用，我们总结了最近的研究集胞藻6803集中在五个主题：细胞培养的限速因素;分子工具的遗传修饰;高通量系统生物学的全基因组分析;代谢模型的生理预测和合理的代谢工程;和生产不同的化学品的应用。我们还讨论了这种微生物的系统分析和工程应用的特殊挑战，包括多功能细胞代谢的精确表征，产品率和滴度的提高，生物工艺放大和产品回收。虽然集胞藻6803作为光养细胞工厂的开发已经取得了很大进展，但“来自集胞藻的化合物”的生物技术仍然明显落后于异养微生物的生物技术（例如，大肠杆菌）。
Cyanobacteria (blue-green algae) play profound roles in ecology and biogeochemistry. One model cyanobacterial species is the unicellular cyanobacterium Synechocystis sp. PCC 6803. This species is highly amenable to genetic modification. Its genome has been sequenced and many systems biology and molecular biology tools are available to study this bacterium. Recently, researchers have put significant efforts into understanding and engineering this bacterium to produce chemicals and biofuels from sunlight and CO2. To demonstrate our perspective on the application of this cyanobacterium as a photosynthesis-based chassis, we summarize the recent research on Synechocystis 6803 by focusing on five topics: rate-limiting factors for cell cultivation; molecular tools for genetic modifications; high-throughput system biology for genome wide analysis; metabolic modeling for physiological prediction and rational metabolic engineering; and applications in producing diverse chemicals. We also discuss the particular challenges for systems analysis and engineering applications of this microorganism, including precise characterization of versatile cell metabolism, improvement of product rates and titers, bioprocess scale-up, and product recovery. Although much progress has been achieved in the development of Synechocystis 6803 as a phototrophic cell factory, the biotechnology for “Compounds from Synechocystis” is still significantly lagging behind those for heterotrophic microbes (e.g., Escherichia coli).
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影响因子：
7.4
作者：
Collins, Aaron M.;Liberton, Michelle;Timlin, Jerilyn A.
通讯作者：
Timlin, Jerilyn A.
DOI：
10.1073/pnas.0600927103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Dühring, U;Axmann, IM;Wilde, A
通讯作者：
Wilde, A
影响因子：
4.1
作者：
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通讯作者：
Kruse, O.
影响因子：
2.2
作者：
Aoki, S;Kondo, T;Ishiura, M
通讯作者：
Ishiura, M
影响因子：
3.2
作者：
Becker, EC;Meyer, RJ
通讯作者：
Meyer, RJ