The influence of lead and arsenite on the inhibition of human breast cancer MCF-7 cell proliferation by American ginseng root (Panax quinquefolius L.)
The influence of lead and arsenite on the inhibition of human breast cancer MCF-7 cell proliferation by American ginseng root (Panax quinquefolius L.)
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DOI：
10.1016/j.lfs.2005.07.010
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发表时间：
2006-02-16
期刊：
影响因子：
6.1
通讯作者：
Murphy, LL
中科院分区：
文献类型：
--
作者：
Corbit, R;Ebbs, S;Murphy, LL
西洋参根（Panax quinquefolius）具有许多据称的治疗效果，包括抑制癌细胞增殖。环境相关的重金属改变人参对癌细胞生长的影响的能力是本研究的主题。西洋参根的水提取物单独或与生理相关剂量的铅（Pb）或亚砷酸盐组合应用于体外MCF-7乳腺癌细胞，并测定对细胞增殖的影响。人参单独从0.5 mg/ml开始对MCF-7细胞增殖产生显著的剂量依赖性抑制。用2.5 μ M亚砷酸盐处理MCF-7细胞显著降低MCF-7细胞增殖（p < 0.01）。当用亚砷酸盐（1.25或2.5 μ M）与人参提取物（0.5 mg/ml）联合处理细胞时，对细胞增殖有明显的协同抑制作用。用50 μ M Pb处理MCF-7乳腺癌细胞，与对照组相比，显著降低细胞增殖（p < 0.01），同时用人参和Pb处理没有导致进一步降低。这些结果表明，污染的重金属，其中一些已被检测到在人参根提取物或商业人参制剂，可能会改变人参的生物活性。(c)2005年爱思唯尔公司All rights reserved.
American ginseng root (Panax quinquefolius) has a number of purported therapeutic effects, including inhibition of cancer cell proliferation. The ability of environmentally relevant heavy metals to alter ginseng effects on cancer cell growth was the subject of this study. A water extract of American ginseng root was applied alone or in combination with physiologically relevant doses of either lead (Pb) or arsenite to MCF-7 breast cancer cells in vitro and effects on cell proliferation were determined. Ginseng alone produced a significant dose-dependent inhibition of MCF-7 cell proliferation starting at 0.5 mg ml(-1). Treatment of MCF-7 cells with 2.5 mu M arsenite significantly decreased MCF-7 cell proliferation (p < 0.01). When cells were treated with arsenite (1.25 or 2.5 mu M) in combination with ginseng extract (0.5 mg ml(-1)), there was an apparent synergistic inhibition of cell proliferation. Treatment of MCF-7 breast cancer cells with 50 mu M Pb significantly decreased cell proliferation relative to control (p < 0.01), and concomitant ginseng and Pb treatment did not lead to a further decrease. These results suggest that contaminant heavy metals, some of which have been detected in ginseng root extracts or commercial ginseng preparations, may alter the biological activity of ginseng. (c) 2005 Elsevier Inc. All rights reserved.