Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.
Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.
复制标题
人类胰岛内分泌细胞亚型的表观遗传学分析。
DOI：
10.1007/978-1-4939-6518-2_8
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kaestner,KlausH
中科院分区：
文献类型：
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作者：
Zhang,Jia;Kaestner,KlausH
表观遗传机制已被提出，有助于多种复杂的疾病，包括糖尿病。解剖正常和疾病状态的表观基因组景观极大地扩展了我们对疾病发病机制中关键参与者的调控的理解，从而为新的治疗途径打开了大门。人胰岛是维持机体葡萄糖平衡的重要微器官。胰岛的异质性阻碍了使用整个胰岛组织进行有意义的深入表观遗传分析，因为一种细胞类型中的重要标记被来自其他细胞类型的信号所掩盖。我们在这里描述了一个详细的协议，高度纯化的内分泌细胞亚型（β，α和δ细胞）从人类尸体胰岛的分离，进行细胞类型特异性表观基因组分析组蛋白修饰以及全球基因表达谱。
Epigenetic mechanisms have been proposed to contribute to multiple complex diseases, including diabetes mellitus. Dissecting the epigenomic landscape of normal and disease states has greatly expanded our understanding of the regulation of key players in disease pathogenesis and is thus opening doors to novel therapeutic avenues. The human pancreatic islet is a pivotal micro-organ that maintains global glucose homeostasis. The heterogeneity of the islet impedes meaningful in-depth epigenetic analysis using whole islet tissue, because important marks in one cell type are masked by signals from other cell types. We describe here a detailed protocol for the isolation of highly purified endocrine cell subtypes (beta, alpha, and delta cells) from human cadaveric islets, to perform cell-type-specific epigenomic analysis of histone modifications as well as global gene expression profiling.