Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants.
Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants.
复制标题
暴露于环境污染物后肝大口黑鲈 (Micropterus salmoides) mRNA 的转录反应。
DOI：
10.1002/jat.1553
复制
发表时间：
2011
期刊：
影响因子：
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通讯作者：
Sepulveda,MariaS
中科院分区：
文献类型：
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作者：
Sanchez,BrianC;Carter,Barbara;Hammers,HeatherR;Sepulveda,MariaS
微阵列可以评估近全基因组中基因转录物表达的变化对环境刺激的反应。我们利用寡核苷酸微阵列和随后的基因集富集分析（GSEA）来评估雄性大口黑鲈（Micropterus salmoides）暴露于常见环境污染物96小时后肝脏组织的基因表达变化模式。以靶体负荷分别为3.0、0.00067、2.5、50和100µg g−1的方式腹腔注射阿特拉津、氯化镉、PCB 126、菲和毒杀芬。这是为了确定暴露的潜在生物标志物。4、126、118、137和58个mRNA转录本的表达分别受到阿特拉嗪、氯化镉、PCB 126、菲和毒虫芬暴露的显著影响（P≤0.001，倍数变化≥2倍）。GSEA显示，暴露于这些化学物质的生物功能基因本体类别分别没有、4个、5个、5个和3个受到显著影响。我们观察到氯化镉引发乙醇代谢反应，并与PCB 126和菲一起影响与蛋白质生物合成相关的转录物。PCB 126、菲和毒虫芬也影响单碳化合物代谢，而PCB 126和菲影响mRNA转录和mRNA从细胞核输出，并可能诱导抗雌激素反应。发现阿特拉津改变了少数肝脏转录物的表达。这项工作强调了几个感兴趣的生物学过程，可能有助于开发鱼类化学暴露的基因转录物生物标志物。版权所有©2010 John Wiley & Sons， Ltd
Microarrays enable gene transcript expression changes in near‐whole genomes to be assessed in response to environmental stimuli. We utilized oligonucleotide microarrays and subsequent gene set enrichment analysis (GSEA) to assess patterns of gene expression changes in male largemouth bass (Micropterus salmoides) hepatic tissues after a 96 h exposure to common environmental contaminants. Fish were exposed to atrazine, cadmium chloride, PCB 126, phenanthrene and toxaphene via intraperitoneal injection with target body burdens of 3.0, 0.00067, 2.5, 50 and 100 µg g−1, respectively. This was conducted in an effort to identify potential biomarkers of exposure. The expressions of 4, 126, 118, 137 and 58 mRNA transcripts were significantly (P≤ 0.001, fold change ≥2×) affected by exposure to atrazine, cadmium chloride, PCB 126, phenanthrene and toxaphene exposures, respectively. GSEA revealed that none, four, five, five and three biological function gene ontology categories were significantly influenced by exposure to these chemicals, respectively. We observed that cadmium chloride elicited ethanol metabolism responses, and along with PCB 126 and phenanthrene affected transcripts associated with protein biosynthesis. PCB 126, phenanthrene and toxaphene also influenced one‐carbon compound metabolism while PCB 126 and phenanthrene affected mRNA transcription and mRNA export from the nucleus and may have induced an antiestrogenic response. Atrazine was found to alter the expression of few hepatic transcripts. This work has highlighted several biological processes of interest that may be helpful in the development of gene transcript biomarkers of chemical exposure in fish. Copyright © 2010 John Wiley & Sons, Ltd.