Clock is important for food and circadian regulation of macronutrient absorption in mice
Clock is important for food and circadian regulation of macronutrient absorption in mice
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DOI：
10.1194/jlr.m900085-jlr200
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发表时间：
2009-09-01
影响因子：
6.5
通讯作者：
Hussain, M. Mahmood
中科院分区：
文献类型：
--
作者：
Pan, Xiaoyue;Hussain, M. Mahmood
生物钟基因对外界刺激做出反应，并表现出昼夜节律。本研究探讨了生物钟基因在小肠中的表达及其在肠上皮细胞营养吸收调控中的作用。我们研究了自由或有限获取食物的野生型和时钟突变（Clk（mt/mt））小鼠小肠中时钟基因和大量营养素转运蛋白的表达。此外，我们研究了这些小鼠对大量营养素的吸收。肠时钟基因表现出昼夜节律的表达，并对野生型小鼠的食物夹带作出反应。Clk（mt/mt）小鼠中的显性负时钟破坏了时钟基因的昼夜节律表达和食物夹带。在Clk（mt/mt）小鼠中，脂质和单糖的吸收较高，而肽的吸收降低。分子研究表明，Clock调节几种参与营养吸收的转运蛋白。生物钟通过调节营养转运蛋白，在肠道功能的光和食物夹带中起重要作用。肠道昼夜节律活动的中断可能导致高脂血症和高血糖症。平移X.，和M. M.侯赛因生物钟对小鼠大量营养素吸收的食物和昼夜节律调节很重要。J. Lipid Res. 2009. 50：1800-1813.
Clock genes respond to external stimuli and exhibit circadian rhythms. This study investigated the expression of clock genes in the small intestine and their contribution in the regulation of nutrient absorption by enterocytes. We examined expression of clock genes and macronutrient transport proteins in the small intestines of wild-type and Clock mutant (Clk(mt/mt)) mice with free or limited access to food. In addition, we studied absorption of macronutrients in these mice. Intestinal clock genes show circadian expression and respond to food entrainment in wild-type mice. Dominant negative Clock in Clk(mt/mt) mice disrupts circadian expression and food entrainment of clock genes. The absorption of lipids and monosaccharides was high in Clk(mt/mt) mice whereas peptide absorption was reduced. Molecular studies revealed that Clock regulates several transport proteins involved in nutrient absorption. Clock plays an important role in light and food entrainment of intestinal functions by regulating nutrient transport proteins. Disruptions in intestinal circadian activity may contribute to hyperlipidemia and hyperglycemia.-Pan, X., and M. M. Hussain. Clock is important for food and circadian regulation of macronutrient absorption in mice. J. Lipid Res. 2009. 50: 1800-1813.